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Evaluation of anti-sperm monoclonal antibodies as biomarkers to assess bull sperm capacitation, acrosome reaction and fertility in vitro Ambrose, Divakar Justus

Abstract

Anti-sperm monoclonal antibodies (mAbs) were evaluated as biomarkers to assess capacitation, acrosome reaction, cryodamage and fertility of bull spermatozoa in vitro. Three anti-human sperm mAbs crossreacting with bull sperm antigens were identified. They showed time-dependent changes in binding to bull spermatozoa incubated under capacitation conditions when assessed by indirect immunofluorescence, with maximum binding at 4 h. Bull and human sperm antigens recognized by one mAb (HS-11) were isolated and their immunological relatedness shown by ELISA. The possible relationship between fertility and the binding of HS- 11 to bull (n=8) spermatozoa was tested in a bovine in vitro fertilization system. In vitro fertility based on cleavage of oocytes and mAb-binding to spermatozoa at 4 h incubation under capacitation conditions were correlated (r=0.43; n=32; P200 kDa in western blots. Three detected more than one protein band (40-200 kDa), while three recognized none. Thirteen of the 15 mAbs were tested for their influence on bovine sperm-zona interactions in vitro. Sperm-zona binding was not affected by 12 mAbs. However, sperm-zona binding in the presence of one surface-reacting mAb was higher than the control (23.6+5.6 vs 10.0+2.4 sperm/zona, mean+SE; P

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