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UBC Theses and Dissertations
Second symmetry in the Balb/c-C57BL/6 (B6) system, and anti-anti-self antibodies in old B6 mice Faheemi, Abdulaziz
Abstract
In order to establish the presence of anti-anti-self antibodies and study such MHC mimicking antibodies in the sera of alloimmune mice, reciprocal sets of alloantisera were raised between the following pairs of mice : Balb/c and C57BL/6 (B6), Balb/c and CBA, and B6 and CBA. After 10 weeks of alloimmunization, the presence of anti-anti-self antibodies could be detected by an avidin-biotin ELISA, and by inhibition of antibody and complement mediated cytotoxicity in the alloimmune B6 and Balb/c sera. Such antibodies belonged to the immunoglobulin G class. Considerably higher levels of anti-antiself antibodies were induced in alloimmune B6 mice compared to alloimmune Balb/c mice. Moreover, anti-anti-self antibodies spontaneously occurred in the serum of old B6 mice. Anti-anti-self antibodies in alloimmune B6 mice were shown to be highly specific. Anti-anti-self antibodies in both alloimmune and old B6 mice specifically inhibited the cytotoxicity of anti-foreign MHC antibodies present in the converse Balb/c anit-B6 antiserum. This indicates that these antibodies have a self MHC mimicking activity.
Item Metadata
Title |
Second symmetry in the Balb/c-C57BL/6 (B6) system, and anti-anti-self antibodies in old B6 mice
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1994
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Description |
In order to establish the presence of anti-anti-self antibodies
and study such MHC mimicking antibodies in the sera of alloimmune
mice, reciprocal sets of alloantisera were raised between the
following pairs of mice : Balb/c and C57BL/6 (B6), Balb/c and CBA,
and B6 and CBA. After 10 weeks of alloimmunization, the presence of
anti-anti-self antibodies could be detected by an avidin-biotin ELISA,
and by inhibition of antibody and complement mediated cytotoxicity
in the alloimmune B6 and Balb/c sera. Such antibodies belonged to
the immunoglobulin G class. Considerably higher levels of anti-antiself
antibodies were induced in alloimmune B6 mice compared to
alloimmune Balb/c mice. Moreover, anti-anti-self antibodies
spontaneously occurred in the serum of old B6 mice. Anti-anti-self
antibodies in alloimmune B6 mice were shown to be highly specific.
Anti-anti-self antibodies in both alloimmune and old B6 mice
specifically inhibited the cytotoxicity of anti-foreign MHC antibodies
present in the converse Balb/c anit-B6 antiserum. This indicates that
these antibodies have a self MHC mimicking activity.
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Extent |
2312677 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-02-24
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0087352
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1994-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.