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UBC Theses and Dissertations
The role for insulin-like growth factor-I in preimplantation embryonic development and decidualization following superovulation in the rat Katagiri, Seiji
Abstract
Superovulation causes detrimental effects, including embryonic loss and implantation failure. This study determined potential roles for insulin-like growth factor (IGF-I) in uterine environment regulation and preimplantation development, in conjunction with the detrimental effects of superovulation in the rat. IGF-I may be beneficial to preimplantation embryonic development. IGF-I stimulated embryonic development and metabolism in vitro and increased the rate of implantation and fetal development when the blastocysts were transferred into a receptive uterus. However, IGF-I may be involved in embryonic loss following superovulation, by perturbing the uterine environment. Superovulation enhanced uterine IGF-I action from day 1 to 3 of pregnancy and reduced its action on days 5 and 6. Uterine luminal fluid from the uterus exposed to increased IGF-I action from day 1 to 3 was detrimental to embryonic development. This uterine luminal fluid had altered electrolyte composition that is similar to that observed following superovulation. Anti-IGF-I antibody restored superovulationinduced alterations in cations, suggesting that IGF-I may partially mediate this effect of superovulation. Superovulation-induced alterations in IGF-I action may adversely affect decidualization, a critical step in implantation. High IGF-I levels from day 1 to 3 and low levels from day 3 to 5, inhibited deciduoma formation. Alterations in IGF-I action after day 5 had no effect, suggesting a role for IGF-I in uterine sensitization. The role of IGF-I in decidualization may be complex. IGF-I cannot be substituted for growth hormone (GH) and thyroxine (T4) during decidualization, but altered deciduoma formation and alkaline phosphatase (ALP) activity in the GH and T4-dependent manner. IGF-I either stimulated or inhibited deciduoma formation and ALP activity, depending upon concentration and treatment period. IGF-I stimulated basal ALP activity but inhibited prostaglandin E2-stimulated ALP activity in the endometrial stroma cells. In conclusion, IGF-I may play an important role in the maintenance of a receptive uterine environment for embryonic development and the regulation of decidualization. Embryonic loss and failure of implantation following superovulation may be partially attributed to disturbances in uterine IGF-I action as observed in this study.
Item Metadata
Title |
The role for insulin-like growth factor-I in preimplantation embryonic development and decidualization following superovulation in the rat
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1996
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Description |
Superovulation causes detrimental effects, including embryonic loss and
implantation failure. This study determined potential roles for insulin-like growth factor
(IGF-I) in uterine environment regulation and preimplantation development, in conjunction
with the detrimental effects of superovulation in the rat.
IGF-I may be beneficial to preimplantation embryonic development. IGF-I
stimulated embryonic development and metabolism in vitro and increased the rate of
implantation and fetal development when the blastocysts were transferred into a receptive
uterus.
However, IGF-I may be involved in embryonic loss following superovulation, by
perturbing the uterine environment. Superovulation enhanced uterine IGF-I action from
day 1 to 3 of pregnancy and reduced its action on days 5 and 6. Uterine luminal fluid from
the uterus exposed to increased IGF-I action from day 1 to 3 was detrimental to embryonic
development. This uterine luminal fluid had altered electrolyte composition that is similar
to that observed following superovulation. Anti-IGF-I antibody restored superovulationinduced
alterations in cations, suggesting that IGF-I may partially mediate this effect of
superovulation.
Superovulation-induced alterations in IGF-I action may adversely affect
decidualization, a critical step in implantation. High IGF-I levels from day 1 to 3 and low
levels from day 3 to 5, inhibited deciduoma formation. Alterations in IGF-I action after
day 5 had no effect, suggesting a role for IGF-I in uterine sensitization.
The role of IGF-I in decidualization may be complex. IGF-I cannot be substituted
for growth hormone (GH) and thyroxine (T4) during decidualization, but altered
deciduoma formation and alkaline phosphatase (ALP) activity in the GH and T4-dependent
manner. IGF-I either stimulated or inhibited deciduoma formation and ALP activity,
depending upon concentration and treatment period. IGF-I stimulated basal ALP activity but inhibited prostaglandin E2-stimulated ALP activity in the endometrial stroma cells.
In conclusion, IGF-I may play an important role in the maintenance of a receptive
uterine environment for embryonic development and the regulation of decidualization.
Embryonic loss and failure of implantation following superovulation may be partially
attributed to disturbances in uterine IGF-I action as observed in this study.
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Extent |
9662964 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-02-19
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0087243
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1996-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.