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Purification and characterization of a selective growth regulator for human myelopoietic progenitor cells

University of British Columbia

A monoclonal antibody, named CAMAL-1, was raised previously in our laboratory to a common antigen of acute myeloid leukemia (CAMAL), and was shown to be highly specific in its recognition of cells from patients with acute (AML) or chronic (CML) myelogenous leukemia. CAMAL was also reported to be prognostic of disease, in that patients whose numbers of CAMAL-1 reactive cells were high, or rose over time, had poorer prognoses and shorter survival times than patients whose CAMAL values were low or decreased. This correlation between CAMAL and disease prognosis led to the discovery that CAMAL-1immunoaffinity-purified leukemic cellular lysates contained a selective growth inhibitory activity for normal myeloid progenitor cells, since the growth of CML progenitors was not inhibited. The work described in this thesis focused primarily on the purification and characterization of the myelopoietic activity present in the CAMAL preparations, and its relationship to the leukemic marker (CAMAL). Initial purifications involved CAMAL-1immunoaffinity chromatography of leukemic cellular lysates, followed by FPLC molecular size fractionation and/or preparative SDS-PAGE. The myelopoietic activity was located within a30-35 kDa molecular weight fraction (P30), and the P30 fraction was consistently found to be selective in its inhibition of normal myeloid progenitors, since the growth of CML progenitors was not inhibited but was, in fact, stimulated. Antibodies were raised to P30 and used in the subsequent purification and characterization of the myelopoietic activity. Amino acid sequence analysis of the N-terminus and P30 tryptic peptides strongly suggested that P30 belonged to the serine protease family of enzymes, and the results obtained from protease assays indicated thatP30 preparations did possess enzyme activity. Prior to the completion of P30 molecular cloning experiments, however, the cDNA sequence for azurocidin/CAP37 was reported, and its predicted amino acid sequence was found to be identical to those obtained from the P30 protein samples. Azurocidin is a proteolytically inactive serine protease homologue, normally present in neutrophilic granules. Purifiedazurocidin did not possess inhibitory activity in normal progenitor cell assays; therefore, in order to isolate the biologic activity from azurocidin and other potentially contaminating proteins, P30 preparations were fractionated by reverse phase HPLC. The rpHPLC profiles were found to be similar to those reported for neutrophilic granules; however, the myelopoietica ctivity was obtained in a single rpHPLC fraction that aligned with the front portion of the azurocidin protein peak. Two dimensional isoelectric focusing/SDS-PAGE analysis of the biologically active rpHPLC fraction confirmed that it contained azurocidin, and no additional protein species were detected. Only the earlier eluting azurocidin rpHPLC fraction mediated the myelopoietic activity, and this fraction was also enriched in the higher molecular weight isoforms of azurocidin. Therefore, it appeared that a variably glycosylated isoform of azurocidin was mediating the biologic effects on myeloid progenitor cells, and because azurocidin obtained from normal neutrophils did not possess the myelopoietic activity, we speculate that the bioactive isoform of azurocidin is present in relatively higher amounts and/or is uniquely synthesized by leukemic cells.

Thesis/Dissertation

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2008-09-18

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http://hdl.handle.net/2429/2237

Microbiology and Immunology

University of British Columbia

UBCV

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