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An action spectrum apparatus Brooks, Donald Elliott

Abstract

An instrument is described which is capable of measuring the action spectrum of the removal of CO inhibition of respiration by light. In the method employed here, a cell suspension in a CO-O₂ atmosphere is alternately exposed to two wavelengths of light. Their photochemical effects are balanced using an 0₂ electrode as the null detector. The light intensities at the balance points from a series of wavelength pairs are used to determine the ratios of the extinction coefficients of the CO - oxidase complex, at the various wavelengths, to the extinction coefficient at a standard wavelength. An action spectrum for Bakers yeast is shown.

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