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Creatine phosphokinase: in vitro activity modification and proteolysis with calpain Arthur, Gavin D.

Abstract

The purpose of this study was to determine if the muscle isoform of the energy transmitting kinase; Creatine Phosphokinase (CPK - EC N° 2.7.3.2) MM-isozyme, is a substrate for calcium activated neutral protease (CANP - EC N° 3.4.22.17). CPK activity was measured under three different conditions: (1) control assay; (2) with 5mM Ca²⁺,(5x10⁻³Ca²⁺j; (3) with 5mM Ca²⁺ and a range of CANP amounts from 10 to lOOug. 5mM Ca²⁺ consistently caused significant inhibition of the CPK activity to 36% of control (p

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