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Determination of exponential rate constants for calpain-mediated degradation of myofibrillar complexed proteins Albisser, Tracie

Abstract

The purpose of this study was to test the hypothesis that an orderly sequence or time course of calpain action on purified myofibrillar/cytoskeletal complexes from cardiac and fast muscle tissue exists. To test the response of individual substrate proteins to degradation (i.e. susceptibility), exogenous calpain (1.5U/ml) was incubated with 40 ug of highly purified rat myofibrillar complexes from cardiac and gastrocnemius muscles (0 to 60 min), in vitro. Apparent molecular weights (SDS-PAGE) were used to compare and identify individual myofibril proteins. Myofibrillar yields for cardiac (n=9) and fast skeletal (n=9) muscles were 62.93 ± 6.58 and 98.42 ± 9.36 mg/g (p

Item Metadata

Title	Determination of exponential rate constants for calpain-mediated degradation of myofibrillar complexed proteins
Creator	Albisser, Tracie
Publisher	University of British Columbia
Date Issued	1996
Description	The purpose of this study was to test the hypothesis that an orderly sequence or time course of calpain action on purified myofibrillar/cytoskeletal complexes from cardiac and fast muscle tissue exists. To test the response of individual substrate proteins to degradation (i.e. susceptibility), exogenous calpain (1.5U/ml) was incubated with 40 ug of highly purified rat myofibrillar complexes from cardiac and gastrocnemius muscles (0 to 60 min), in vitro. Apparent molecular weights (SDS-PAGE) were used to compare and identify individual myofibril proteins. Myofibrillar yields for cardiac (n=9) and fast skeletal (n=9) muscles were 62.93 ± 6.58 and 98.42 ± 9.36 mg/g (p
Extent	6687849 bytes
Genre	Thesis/Dissertation
Type	Text
File Format	application/pdf
Language	eng
Date Available	2009-03-07
Provider	Vancouver : University of British Columbia Library
Rights	For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
DOI	10.14288/1.0077157
URI	http://hdl.handle.net/2429/5726
Degree	Master of Science - MSc
Program	Human Kinetics
Affiliation	Education, Faculty of; Kinesiology, School of
Degree Grantor	University of British Columbia
Graduation Date	1997-05
Campus	UBCV
Scholarly Level	Graduate
Aggregated Source Repository	DSpace

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ubc_1997-0053.pdf -- 6.38MB

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For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.