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Determination of exponential rate constants for calpain-mediated degradation of myofibrillar complexed proteins Albisser, Tracie
Abstract
The purpose of this study was to test the hypothesis that an orderly sequence or time course of calpain action on purified myofibrillar/cytoskeletal complexes from cardiac and fast muscle tissue exists. To test the response of individual substrate proteins to degradation (i.e. susceptibility), exogenous calpain (1.5U/ml) was incubated with 40 ug of highly purified rat myofibrillar complexes from cardiac and gastrocnemius muscles (0 to 60 min), in vitro. Apparent molecular weights (SDS-PAGE) were used to compare and identify individual myofibril proteins. Myofibrillar yields for cardiac (n=9) and fast skeletal (n=9) muscles were 62.93 ± 6.58 and 98.42 ± 9.36 mg/g (p
Item Metadata
Title |
Determination of exponential rate constants for calpain-mediated degradation of myofibrillar complexed proteins
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1996
|
Description |
The purpose of this study was to test the hypothesis that an orderly sequence or
time course of calpain action on purified myofibrillar/cytoskeletal complexes from cardiac
and fast muscle tissue exists. To test the response of individual substrate proteins to
degradation (i.e. susceptibility), exogenous calpain (1.5U/ml) was incubated with 40 ug of
highly purified rat myofibrillar complexes from cardiac and gastrocnemius muscles (0 to
60 min), in vitro. Apparent molecular weights (SDS-PAGE) were used to compare and
identify individual myofibril proteins. Myofibrillar yields for cardiac (n=9) and fast skeletal
(n=9) muscles were 62.93 ± 6.58 and 98.42 ± 9.36 mg/g (p
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Extent |
6687849 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-03-07
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0077157
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1997-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.