- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Theses and Dissertations /
- Sequencing of p53 genes from Mytilus edulis and Mytilus...
Open Collections
UBC Theses and Dissertations
UBC Theses and Dissertations
Sequencing of p53 genes from Mytilus edulis and Mytilus trossulus for use in environmental effects monitoring of primary treated effluent Muttray, Annette Friederike
Abstract
This thesis describes the sequencing and potential use of a genetic biomarker for coastal environmental effects monitoring of municipal effluents. The Blue Mussel {Mytilus edulis) and the Bay Mussel (Mytilus trossulus) are currently investigated for their use in municipal effluent effects monitoring. Both can develop a disease of the haemolymph, called haemic neoplasia or leukemia, albeit at different prevalences. The p53 gene is a tumor suppressor gene that is fundamental in cell cycle control and apoptosis. It is mutated or differentially expressed in about 50% of all human cancers and has been implicated in leukemia development in clams. Thus, the p53 gene family was chosen as potential biomarkers for haemic neoplasia in Mytilus spp. During this study, the p53 mRNA sequences of both Mytilus species were elucidated and analyzed in detail using several databases. Sequences show 99.8% similarity on the protein level, but are only 96.5% similar at the DNA level, and differ especially in their 3 untranslated regions, which are important in the regulation of post-transcriptional events. Future studies are required to show potential linkages of p53 gene family expression patterns with haemic neoplasia in the mussels. During this study, an additional gene sequence was discovered and is submitted as a Mytilin C antimicrobial peptide precursor in M. trossulus. These peptides occur in the haemolymph and haemocytes of mussels and prevent microbial infections of the open circulatory system. This thesis also reviews the current state of knowledge on the use of molecular biomarkers for monitoring effects of endocrine disrupting compounds and surfactants and reviews iri-depth the fate and effects of one group of surfactants, linear alkylbenzene sulfonates (LAS). LAS were identified as a major cause of toxicity in the effluent of one treatment plant in the Greater Vancouver Regional District, and have been shown to enhance the effects of endocrine disrupting compounds. Based on our current knowledge about increased prevalence of haemic neoplasia in other bivalves in polluted areas, clam p53 gene family expression patterns, as well as environmental carcinogenesis involving the endocrine and p53 systems in humans, it is suggested that the p53 gene family is a good candidate for development of a molecular biomarker.
Item Metadata
Title |
Sequencing of p53 genes from Mytilus edulis and Mytilus trossulus for use in environmental effects monitoring of primary treated effluent
|
Creator | |
Publisher |
University of British Columbia
|
Date Issued |
2004
|
Description |
This thesis describes the sequencing and potential use of a genetic biomarker for
coastal environmental effects monitoring of municipal effluents. The Blue Mussel
{Mytilus edulis) and the Bay Mussel (Mytilus trossulus) are currently investigated for
their use in municipal effluent effects monitoring. Both can develop a disease of the
haemolymph, called haemic neoplasia or leukemia, albeit at different prevalences. The
p53 gene is a tumor suppressor gene that is fundamental in cell cycle control and
apoptosis. It is mutated or differentially expressed in about 50% of all human cancers
and has been implicated in leukemia development in clams. Thus, the p53 gene family
was chosen as potential biomarkers for haemic neoplasia in Mytilus spp. During this
study, the p53 mRNA sequences of both Mytilus species were elucidated and analyzed
in detail using several databases. Sequences show 99.8% similarity on the protein level, but are only 96.5% similar at the DNA level, and differ especially in their 3 untranslated regions, which are important in the regulation of post-transcriptional events. Future studies are required to show potential linkages of p53 gene family expression patterns with haemic neoplasia in the mussels. During this study, an
additional gene sequence was discovered and is submitted as a Mytilin C antimicrobial
peptide precursor in M. trossulus. These peptides occur in the haemolymph and haemocytes of mussels and prevent microbial infections of the open circulatory system. This thesis also reviews the current state of knowledge on the use of molecular
biomarkers for monitoring effects of endocrine disrupting compounds and surfactants and reviews iri-depth the fate and effects of one group of surfactants, linear alkylbenzene sulfonates (LAS). LAS were identified as a major cause of toxicity in the effluent of one treatment plant in the Greater Vancouver Regional District, and have been shown to enhance the effects of endocrine disrupting compounds. Based on our
current knowledge about increased prevalence of haemic neoplasia in other bivalves in
polluted areas, clam p53 gene family expression patterns, as well as environmental
carcinogenesis involving the endocrine and p53 systems in humans, it is suggested that
the p53 gene family is a good candidate for development of a molecular biomarker.
|
Extent |
15474995 bytes
|
Genre | |
Type | |
File Format |
application/pdf
|
Language |
eng
|
Date Available |
2009-11-25
|
Provider |
Vancouver : University of British Columbia Library
|
Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
|
DOI |
10.14288/1.0063442
|
URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
|
Graduation Date |
2004-11
|
Campus | |
Scholarly Level |
Graduate
|
Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.