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UBC Theses and Dissertations
Effects of additives on analyte migration behavior in capillary electrophoresis Kranack, Andrea R.
Abstract
The aim of this work is to study the effects of additives on the analyte migration behavior in capillary electrophoresis (CE). The first part of this thesis presents an investigation into the interaction between the analytes and the additives in both the liquid and gas phase. ß-Cyclodextrin (ß-CD) was used as the additive and several nitrophenols were employed as analytes to compare the trends of the binding affinity in the gas and liquid phase. It was demonstrated that the trend of the equilibrium constants (K) in the aqueous phase determined by CE was the same as in the gaseous phase determined by electrospray mass spectrometry. It was also concluded that ammonium ions were bound to (ß-CD in the gas phase. The second part of this thesis presents a quantitative study of ion-pairing interactions in CE. The ion-pairing agents dodecyltrimethylammonium bromide (DTAB) and tetramethylarnmonium bromide (TMAB) were used as additives in several separation buffers to examine the relationship between the analytes and the additives in aqueous and nonaqueous CE. It was concluded that the interaction between the analytes and the additives in this study was mainly due to ion-pairing and that hydrophobic interactions played a minor role. In the final part of this thesis, the effects of multicomponent additives were studied. Sulfobutylether-ß-CD (SBE-ß-CD) and hydroxypropyl-ß-CD (HP-PCD) were used as differently charged additives. Once the interaction between the analytes with each additive was understood, the effects of a mixture of the two differently charged additives were examined.
Item Metadata
Title |
Effects of additives on analyte migration behavior in capillary electrophoresis
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1998
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Description |
The aim of this work is to study the effects of additives on the analyte migration behavior in
capillary electrophoresis (CE). The first part of this thesis presents an investigation into the
interaction between the analytes and the additives in both the liquid and gas phase. ß-Cyclodextrin
(ß-CD) was used as the additive and several nitrophenols were employed as
analytes to compare the trends of the binding affinity in the gas and liquid phase. It was
demonstrated that the trend of the equilibrium constants (K) in the aqueous phase determined by
CE was the same as in the gaseous phase determined by electrospray mass spectrometry. It was
also concluded that ammonium ions were bound to (ß-CD in the gas phase. The second part of
this thesis presents a quantitative study of ion-pairing interactions in CE. The ion-pairing agents
dodecyltrimethylammonium bromide (DTAB) and tetramethylarnmonium bromide (TMAB) were
used as additives in several separation buffers to examine the relationship between the analytes
and the additives in aqueous and nonaqueous CE. It was concluded that the interaction between
the analytes and the additives in this study was mainly due to ion-pairing and that hydrophobic
interactions played a minor role. In the final part of this thesis, the effects of multicomponent
additives were studied. Sulfobutylether-ß-CD (SBE-ß-CD) and hydroxypropyl-ß-CD (HP-PCD)
were used as differently charged additives. Once the interaction between the analytes with
each additive was understood, the effects of a mixture of the two differently charged additives
were examined.
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Extent |
5398540 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-04-30
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0061576
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1998-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.