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UBC Theses and Dissertations

The development of novel applications of Raman spectroscopy Bass, Adam Jason

Abstract

This thesis explores two different facets of Raman spectroscopy that aim to extend the applications of this technique. Ultraviolet Resonance Raman Spectroscopy was used to examine the stable tryptophan and tyrosine radicals of the reaction of Cytochrome c Peroxidase (CcP) with Hydrogen Peroxide. Native CcP, reduced CcP and a CcP W191F mutant were analyzed at 248.2 nm in a fibre-optic probe setup and difference spectra were obtained from the data. Signals seen in the difference spectra were attributed to the tryptophan and tyrosine radicals in the protein. A surface plasmon resonance (SPR) imager was designed and built that interfaced with a Renishaw 1000B microscope with the future aim of analyzing DNA microarrays. The combination of the two techniques aimed to harness the enhancement advantages of Surface Enhanced Raman Spectroscopy (SERS). The device successfully imaged liquid drops on it's surface and was able to distinguish differences of refractive index between the solution of as little as 5x10⁻³. The spectrum of evaporated Salmon Sperm DNA spotted on the surface of the SPR imager was collected and a mild enhancement of the spectrum was observed when the SPR imager was operating concurrently during the acquisition of Raman spectra.

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