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UBC Theses and Dissertations

The effect of manganese on the concentration of biologically available copper to the diatom, Thalassiosira pseudonana Kazumi, Junko


Mn was found to reduce the toxicity of Cu to the marine diatom Thalassiosira pseudonana (clone 3H) in the chemically well defined medium Aquil (Morel et al., 1979), verifying the results of Sunda and Huntsman (1983). A cation-exchange resin technique developed by Zorkin (1983) was modified for use in natural seawater samples to estimate the biologically active Cu and Mn. Seawater samples taken from the bottom waters of a local fjord were found to support better growth of the test organism than samples from shallow waters, although the concentration of the biologically active Cu as estimated by the resin technique was similar for samples collected from all depths. The biologically active Mn concentration was found to be higher in the bottom water samples, indicating that the bioassay organism was probably responding to the interaction between the ionic forms of Cu and Mn, rather than to changes in the biologically active Cu concentration.

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