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The role of riboflavin in vascular health Ricci, Taylor Annelia
Abstract
Background: Endothelial cells are modulate vascular tone and interface with systemic circulation. Riboflavin is a B-vitamin that is involved in redox and energy metabolism as flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN). FAD/FMN are also required for the synthesis of vasodilatory nitric oxide (NO) by endothelial nitric oxide synthase (eNOS). However, the role of riboflavin in endothelial NO production and vascular homeostasis is not understood. The objective of my thesis was to determine the role of riboflavin on vascular function. Methods: Male and female C57BL/6J Nos3-/- mice (homozygous eNOS knockout) and Nos3+/+ littermates were fed a control diet (6mg/kg riboflavin) or low riboflavin (1mg/kg riboflavin) diet from weaning for 8 weeks (n=5-7 mice/sex/diet/genotype). Thoracic aorta was collected to assess ex vivo vascular endothelial-dependent and independent function, intima media thickness, and endothelial gene expression. Serum was collected for analysis of one-carbon metabolites. Results: Male and female mice fed the low riboflavin diet had lower (p
Item Metadata
Title |
The role of riboflavin in vascular health
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Creator | |
Supervisor | |
Publisher |
University of British Columbia
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Date Issued |
2025
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Description |
Background: Endothelial cells are modulate vascular tone and interface with systemic circulation. Riboflavin is a B-vitamin that is involved in redox and energy metabolism as flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN). FAD/FMN are also required for the synthesis of vasodilatory nitric oxide (NO) by endothelial nitric oxide synthase (eNOS). However, the role of riboflavin in endothelial NO production and vascular homeostasis is not understood. The objective of my thesis was to determine the role of riboflavin on vascular function.
Methods: Male and female C57BL/6J Nos3-/- mice (homozygous eNOS knockout) and Nos3+/+ littermates were fed a control diet (6mg/kg riboflavin) or low riboflavin (1mg/kg riboflavin) diet from weaning for 8 weeks (n=5-7 mice/sex/diet/genotype). Thoracic aorta was collected to assess ex vivo vascular endothelial-dependent and independent function, intima media thickness, and endothelial gene expression. Serum was collected for analysis of one-carbon metabolites.
Results: Male and female mice fed the low riboflavin diet had lower (p
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Genre | |
Type | |
Language |
eng
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Date Available |
2025-02-18
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Provider |
Vancouver : University of British Columbia Library
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Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
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DOI |
10.14288/1.0448087
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URI | |
Degree (Theses) | |
Program (Theses) | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
2025-05
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Campus | |
Scholarly Level |
Graduate
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Rights URI | |
Aggregated Source Repository |
DSpace
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Rights
Attribution-NonCommercial-NoDerivatives 4.0 International