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UBC Theses and Dissertations

The role of CSF1R in early tooth development Nagra, Ashina


Objective: Colony stimulating factor 1 receptor (CSF1R) binds its ligand CSF1 and regulates the formation of osteoclasts. Mice nullizgyous for CSF1 (Csf1op/op) or CSF1R (Csf1r-/-) fail to erupt teeth due to defective osteoclast formation. Dental abnormalities have also been observed postnatally in the unerupted teeth of these mouse models. However, it is not understood if these dental abnormalities are caused by direct effects of CSF1R or defects in bone remodeling. Therefore, the aim of this thesis was to identify a direct role of CSF1R in early tooth development. Methods: Immunostaining techniques were used to show CSF1R expression during early stages of odontogenesis. CSF1R was inhibited in utero during key odontogenic stages. Teeth from embryos and offspring were collected at different ages and studied for phenotypic abnormalities using histological techniques. Teeth from adult mice were analyzed using high-resolution micro-computed tomography. Results: CSF1R expression was found in ectomesenchymal tissues around normally developing incisors and molars. Osteoclasts had similar localization patterns. Prenatal CSF1R inhibition during odontogenesis led to significant dental abnormalities observed prenatally and postnatally due to altered bone remodeling. Ultimately, continuous tooth growth of mouse incisors postnatally was not impacted. Conclusion: These results indicate that CSF1R regulates tooth morphology during the bell stage, likely by remodeling the bony crypts at the tooth-bone interface.

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