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Antibacterial effect of extract from commiphora gileadensis ; in vitro study Abuljadayel, Khalid

Abstract

Objective: This in vitro study aims to investigate the antimicrobial effect of extracts from Commiphora gileadensis (C. gileadensis) on one and three weeks old oral anaerobic multispecies biofilms and to compare them to 2% chlorhexidine (CHX). Material and Methods: Hydroxyapatite discs were coated with type I collagen and immersed in Brain Heart Infusion broth infused with an oral subgingival plaque obtained from two donors. The discs were then incubated under anaerobic conditions for one or three weeks. After biofilm growth, the discs were exposed to one or three minutes for the following solutions: 1 - C. gileadensis 1mg/ml water, 2 - C. gileadensis 0.1mg/ml water, 3 - C. gileadensis 1mg/ml 0.5% DMSO, 4 - C. gileadensis 0.1mg/ml 0.5% DMSO, 5 - water, 6 - 2%CHX and 7 - 0.5%DMSO. After the exposures, all discs were stained with a viability stain and scanned under a confocal laser scanning microscope. The percentage of dead bacteria was calculated using Imaris software. The data were submitted to Univariate analysis of variance and Tukey statistical tests (P < 0.05). Results: C. gileadensis 1mg/ml water killed significantly more bacteria than all other groups (P < 0.05). 2%CHX and other groups of C. gileadensis killed bacteria without a significant difference among themselves (P > 0.05). In 0.5%DMSO and water groups only a few bacteria were killed. Conclusion: Commiphora gileadensis extract 1mg/ml in water killed significantly more bacteria in oral anaerobic multispecies biofilm than 2% CHX.

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