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UBC Theses and Dissertations
UBC Theses and Dissertations
Terpenoids from terrestrial and marine sources Hirkala-Schaefer, Truman
Abstract
The prehistoric Australian conifer Wollemia nobilis was thought to be extinct until the discovery of living specimens in 1992. Due to its threatened status and restricted distribution, investigations into its metabolic constituents have been limited. Chapter 2 details the phytochemical investigation of Wollemia nobilis. Crude organic extracts from the leaves Wollemia nobilis collected on UBC campus were screened for bioactivity against a series of pathogens. Bioassay guided fractionation led to the isolation and structural elucidation of the novel compound 2.12, which upon examination of the experimental data was found to be the true structure of the previously reported compound wollemolide (2.11). Additionally, two new endoperoxide diterpenoids (2.14 & 2.15), several known compounds new to the Wollemia genus (2.13 & 2.16-2.18), as well as sandaracopimaric acid (2.4) previously reported from Wollemia nobilis were isolated and structurally elucidated. The pure compounds demonstrated no in vitro activity against the screened pathogens Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Candida albicans and methicillin resistant Staphylococcus aureus (MRSA). The Indonesian sponge Petrosia contignata has been the source of several novel bioactive metabolites. Though previously examined, the crude sponge extracts of Petrosia contignata were investigated for less abundant metabolites. Chapter 3 describes the isolation and structural elucidation of the new tetrahydroxylated steroid petrosiasterol (3.9). as well as three known steroids new to the Petrosia genus (3.7, 3.8, & 3.11) and one previously reported from the Petrosia genus (3.10).
Item Metadata
Title |
Terpenoids from terrestrial and marine sources
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
2020
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Description |
The prehistoric Australian conifer Wollemia nobilis was thought to be extinct until the discovery of living specimens in 1992. Due to its threatened status and restricted distribution, investigations into its metabolic constituents have been limited. Chapter 2 details the phytochemical investigation of Wollemia nobilis. Crude organic extracts from the leaves Wollemia nobilis collected on UBC campus were screened for bioactivity against a series of pathogens. Bioassay guided fractionation led to the isolation and structural elucidation of the novel compound 2.12, which upon examination of the experimental data was found to be the true structure of the previously reported compound wollemolide (2.11). Additionally, two new endoperoxide diterpenoids (2.14 & 2.15), several known compounds new to the Wollemia genus (2.13 & 2.16-2.18), as well as sandaracopimaric acid (2.4) previously reported from Wollemia nobilis were isolated and structurally elucidated. The pure compounds demonstrated no in vitro activity against the screened pathogens Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Candida albicans and methicillin resistant Staphylococcus aureus (MRSA).
The Indonesian sponge Petrosia contignata has been the source of several novel bioactive metabolites. Though previously examined, the crude sponge extracts of Petrosia contignata were investigated for less abundant metabolites. Chapter 3 describes the isolation and structural elucidation of the new tetrahydroxylated steroid petrosiasterol (3.9). as well as three known steroids new to the Petrosia genus (3.7, 3.8, & 3.11) and one previously reported from the Petrosia genus (3.10).
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Genre | |
Type | |
Language |
eng
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Date Available |
2021-04-30
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Provider |
Vancouver : University of British Columbia Library
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Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
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DOI |
10.14288/1.0389990
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
2020-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Rights
Attribution-NonCommercial-NoDerivatives 4.0 International