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Understanding the role of iron in biofilm formation, characterization, and viable but non-culturable state induction and resuscitation in Salmonella spp. Pasquale, Deanna Marie
Abstract
Non-typhoidal strains of Salmonella spp. are one of the greatest bacterial contributors to food-borne illness in Canada. Until the 1990’s, reports of food-borne illness were mainly associated with contaminated meat products; however, raw or minimally cooked produce is now being identified as a major source of food-borne outbreaks. Salmonella are able to persist on crop plants through biofilm formation, and existing in a viable but non culturable (VBNC) state; currently, little is known about the effect of iron on these processes. Using wildtype and iron mutants of Salmonella Typhimurium and Salmonella Enteritidis, the effect of iron was investigated. The first objective was to determine the effect of iron on Salmonella biofilm formation in vitro. Crystal violet (CV) biofilm assays, cell surface hydrophobicity assays, aggregation assays, motility, Congo red (CR) and calcofluor plates were used to characterize and quantify biofilm formation. In vitro work showed that the S. Typhimurium ton B mutant had significantly (p
Item Metadata
Title |
Understanding the role of iron in biofilm formation, characterization, and viable but non-culturable state induction and resuscitation in Salmonella spp.
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
2019
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Description |
Non-typhoidal strains of Salmonella spp. are one of the greatest bacterial contributors to food-borne illness in Canada. Until the 1990’s, reports of food-borne illness were mainly associated with contaminated meat products; however, raw or minimally cooked produce is now being identified as a major source of food-borne outbreaks. Salmonella are able to persist on crop plants through biofilm formation, and existing in a viable but non culturable (VBNC) state; currently, little is known about the effect of iron on these processes. Using wildtype and iron mutants of Salmonella Typhimurium and Salmonella Enteritidis, the effect of iron was investigated.
The first objective was to determine the effect of iron on Salmonella biofilm formation in vitro. Crystal violet (CV) biofilm assays, cell surface hydrophobicity assays, aggregation assays, motility, Congo red (CR) and calcofluor plates were used to characterize and quantify biofilm formation. In vitro work showed that the S. Typhimurium ton B mutant had significantly (p
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Genre | |
Type | |
Language |
eng
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Date Available |
2019-05-01
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Provider |
Vancouver : University of British Columbia Library
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Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
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DOI |
10.14288/1.0378556
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URI | |
Degree (Theses) | |
Program (Theses) | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
2019-05
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Campus | |
Scholarly Level |
Graduate
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Rights URI | |
Aggregated Source Repository |
DSpace
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Rights
Attribution-NonCommercial-NoDerivatives 4.0 International