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Evaluation of a ligand-directed labeling molecule targeting integrin alphaVbeta5

University of British Columbia

Integrins are transmembrane receptors that mediate cellular adhesion to the extracellular matrix and initiate signaling cascades that may be relevant to synaptic elimination events occurring in neurodegenerative disease. To study the role of integrins in synaptic elimination, a novel ligand-directed labeling molecule has been created, comprised of the Pacific Blue™ (PB) fluorophore and the high-affinity integrin inhibitor, cilengitide. It was hypothesized that the function of integrin αVβ5 could be studied in live cells by using this probe to track subcellular integrin localization through fluorescence microscopy. This study evaluated the pharmacodynamic properties of the PB-probe, finding that its fluorescence excitation and emission properties are similar to PB and highly photostable over the time period of at least 2 h. Fluorescence enhancement and anisotropy experiments revealed that a derivative of the probe binds integrin αVβ5 with a dissociation constant of 0.11 ± 0.07 μM. Binding was competitively inhibited by cilengitide, suggesting that both molecules compete for the integrin binding domain with similar affinities. Application to BV-2 murine microglia in vitro confirmed that the PB-probe is a robust and rapidly applicable labeling method for the fluorescence detection of endogenous integrins. These experiments demonstrated that PB-probe labeling is specific, amenable to a variety of sample preparation methods, and compatible with simultaneous immunocytochemistry techniques. Separately, the PB-probe was found to inhibit integrin-mediated signaling cascades with equal efficacy as cilengitide. The results of this study confirm that the PB-probe retains both similar fluorescent properties as PB and the integrin-binding properties of cilengitide. These findings establish the PB-probe as an effective means of tracking endogenous integrin αVβ5 in live-cell systems, representing a critical first step in understanding the role of integrin signaling in synaptic elimination.

Text

2018-12-13

Attribution-NonCommercial-NoDerivatives 4.0 International

http://hdl.handle.net/2429/68069

Biochemistry and Molecular Biology

University of British Columbia

UBCO

http://creativecommons.org/licenses/by-nc-nd/4.0/