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UBC Theses and Dissertations

Determination of thiabendazole in orange juice using an MISPE-SERS chemosensor Feng, Jingyi


Thiabendazole is a systemic fungicide frequently used to treat vegetables and fruits during the post-harvest process, leading to potential detriment to consumers. This work has combined molecularly imprinted polymers (MIPs) with surface enhanced Raman spectroscopy (SERS) to afford a novel MISPE-SERS chemosensor to determine trace amount of thiabendazole in orange juice. MIPs were synthesized via precipitation polymerization. Kinetic and static adsorption tests validated the efficient separation and enrichment of thiabendazole by using MIPs. Synthesized MIPs were packed into solid phase extraction (SPE) cartridge to work as tailor-made sorbents for the separation of thiabendazole in orange juice. Silver colloid functioned as SERS-active substrate to quantify eluted thiabendazole by MISPE-SERS chemosensor. The whole process only took 23 min with limit of detection of 4 ppm for thiabendazole. In sum, this MISPE-SERS chemosensor can be considered as a promising method for fast and high-throughput detection of trace amount of thiabendazole in agri-food products.

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