UBC Theses and Dissertations
Impact of y14 phosphorylation of caveolin-1 on its binding partners : a proteomic analysis Saxena, Sandeep
Caveolae, a special type of lipid-raft, are cave-like invaginations of plasma-membrane maintained and formed by Caveolins (Cav1, 2 and 3) and Cavin 1 and 2. Caveolae regulate various trafficking and signaling pathways. Cav1, a 178 amino acid protein has a Src-dependent tyrosine-14 phosphorylation site that regulates integrin signaling and focal adhesion dynamics, and a Caveolin Scaffolding Domain (CSD) that physically interacts with multiple proteins. Glutathione-S-transferase (GST) pull-downs and quantitative proteomics analysis (Maxquant) were performed using lysates from the DU145 prostate cancer cell line and GST-beads tagged with the N-terminal polypeptide of Cav1 (amino acids 1-101) incorporating phosphomimeitic (Y14D) and non-phosphorylatable (Y14F) mutations. Proteomic analysis showed 1.5 fold increased interaction of 196 and 78 proteins with Cav1(1-101)Y14D and Cav1(1-101)Y14F, respectively. Gene Ontology (GO) analysis revealed that Cav1(1-101)Y14D interacted more with proteins that regulate cell stress, proliferation, signal transduction, metabolic processes, apoptosis (Heat shock protein-90 (HSP90)) and focal adhesions, whereas Cav1(1-101)Y14F interacted more with proteins that regulate actin cytoskeleton and RNA processing. Pseudopod-enriched proteome list from DU145 cells revealed 84 proteins overlapping with the Cav1(1-101)Y14D interactome. Comparative proteomics analysis of the CSD mutants (F92A/V94A) suggested that one-third binding proteins of Cav1(1-101)Y14D and Cav1(1-101)Y14F were influenced by this mutation. Binding specificity of Y14 phosphorylation on Cav1 is partially affected by these CSD mutations. Pseudopod enriched HSP90 was one of the top hits in the Cav1(1-101)Y14D interactome. Inhibition of HSP90 with 17-N-allylamino-17-demethoxygeldanamycin (17AAG) increased expression of Protein Kinase B (also known as AKT) and Cav1 in pCav1 (Y14 phosphorylatedCav1) expressing DU145 and PC3 prostate cancer cell lines. However, there was no effect of HSP90 inhibition on pCav1 lacking DU145 and Cav1 knocked-down PC3 cells. Reduced cell migration and viability was observed after 17AAG treatment of DU145 (stably expressing various Cav1 mutants) and PC3 in pCav1 dependent manner. This suggests the HSP90 function in regulating cell migration rely on pCav1. This study reveals that Y14 phosphorylation impacts Cav1 interaction with different proteins and is partially affected by CSD mutants in our study. Also, pCav1 specific interaction with HSP90 decreases prostate cancer cell migration.
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