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Investigation of the presence of GnRH and GnRH-R system in bovine oocytes, sperm and early embryos and their functional role in reproduction Perera, Uswatteliyanaralalage Felicia Ruwanie
Abstract
The objectives of this study were to investigate: 1) the effect of GnRH agonist on oocyte maturation, sperm function and fertilization, 2) the presence of GnRH-R in bovine oocytes, sperm and early embryos. To examine the effect of GnRH agonist on sperm function, sperm were incubated in modified Tyrode’s medium with 0, 0.2, 0.4, 0.8 and 1.2 µgmL⁻¹ of buserelin and with1 µgmL⁻¹ P₄ for 3 h. Acrosome status in each group was assessed at 0 h and 3 h. For zona-binding assay, in vitro matured oocytes were co-incubated with sperm in different concentrations of buserelin and P₄ for 4 h and the zona-bound sperm in each treatment group was determined. Acrosome reacted percentage were higher in sperm treated with 0.4, 0.8 µgmL⁻¹ buserelin than the control group (p
Item Metadata
| Title |
Investigation of the presence of GnRH and GnRH-R system in bovine oocytes, sperm and early embryos and their functional role in reproduction
|
| Creator | |
| Publisher |
University of British Columbia
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| Date Issued |
2010
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| Description |
The objectives of this study were to investigate: 1) the effect of GnRH agonist on oocyte maturation, sperm function and fertilization, 2) the presence of GnRH-R in bovine oocytes, sperm and early embryos.
To examine the effect of GnRH agonist on sperm function, sperm were incubated in modified Tyrode’s medium with 0, 0.2, 0.4, 0.8 and 1.2 µgmL⁻¹ of buserelin and with1 µgmL⁻¹ P₄ for 3 h. Acrosome status in each group was assessed at 0 h and 3 h. For zona-binding assay, in vitro matured oocytes were co-incubated with sperm in different concentrations of buserelin and P₄ for 4 h and the zona-bound sperm in each treatment group was determined. Acrosome reacted percentage were higher in sperm treated with 0.4, 0.8 µgmL⁻¹ buserelin than the control group (p
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| Genre | |
| Type | |
| Language |
eng
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| Date Available |
2010-04-06
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| Provider |
Vancouver : University of British Columbia Library
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| Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
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| DOI |
10.14288/1.0069546
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| URI | |
| Degree (Theses) | |
| Program (Theses) | |
| Affiliation | |
| Degree Grantor |
University of British Columbia
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| Graduation Date |
2010-05
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| Campus | |
| Scholarly Level |
Graduate
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| Rights URI | |
| Aggregated Source Repository |
DSpace
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Rights
Attribution-NonCommercial-NoDerivatives 4.0 International