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UBC Theses and Dissertations

Investigation of the presence of GnRH and GnRH-R system in bovine oocytes, sperm and early embryos and their functional role in reproduction Perera, Uswatteliyanaralalage Felicia Ruwanie

Abstract

The objectives of this study were to investigate: 1) the effect of GnRH agonist on oocyte maturation, sperm function and fertilization, 2) the presence of GnRH-R in bovine oocytes, sperm and early embryos. To examine the effect of GnRH agonist on sperm function, sperm were incubated in modified Tyrode’s medium with 0, 0.2, 0.4, 0.8 and 1.2 µgmL⁻¹ of buserelin and with1 µgmL⁻¹ P₄ for 3 h. Acrosome status in each group was assessed at 0 h and 3 h. For zona-binding assay, in vitro matured oocytes were co-incubated with sperm in different concentrations of buserelin and P₄ for 4 h and the zona-bound sperm in each treatment group was determined. Acrosome reacted percentage were higher in sperm treated with 0.4, 0.8 µgmL⁻¹ buserelin than the control group (p

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Attribution-NonCommercial-NoDerivatives 4.0 International