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An Amidase Contributes to Full Virulence of Sclerotinia sclerotiorum Li, Wei; Lu, Junxing; Yang, Chenghuizi; Arildsen, Kate; Li, Xin; Xia, Shitou

Abstract

Sclerotinia sclerotiorum is one of the most notorious and ubiquitous soilborne plant pathogens, causing serious economic losses to a large number of hosts worldwide. Although virulence factors have been identified in this filamentous fungus, including various cell-wall-degrading enzymes, toxins, oxalic acids and effectors, our understanding of its virulence strategies is far from complete. To explore novel factors contributing to disease, a new pipeline combining forward genetic screening and next-generation sequencing was utilized in this study. Analysis of a hypovirulent mutant revealed that a mutation in an amidase-encoding gene, Sscle_10g079050, resulted in reduced virulence. This is a first report on the contribution of an amidase to fungal virulence, likely through affecting oxalic acid homeostasis.

Item Metadata

Title
An Amidase Contributes to Full Virulence of Sclerotinia sclerotiorum
Creator
Li, Wei; Lu, Junxing; Yang, Chenghuizi; Arildsen, Kate; Li, Xin; Xia, Shitou
Contributor
Michael Smith Laboratories
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2022-09-23
Description
Sclerotinia sclerotiorum is one of the most notorious and ubiquitous soilborne plant pathogens, causing serious economic losses to a large number of hosts worldwide. Although virulence factors have been identified in this filamentous fungus, including various cell-wall-degrading enzymes, toxins, oxalic acids and effectors, our understanding of its virulence strategies is far from complete. To explore novel factors contributing to disease, a new pipeline combining forward genetic screening and next-generation sequencing was utilized in this study. Analysis of a hypovirulent mutant revealed that a mutation in an amidase-encoding gene, Sscle_10g079050, resulted in reduced virulence. This is a first report on the contribution of an amidase to fungal virulence, likely through affecting oxalic acid homeostasis.
Subject
Sclerotinia sclerotiorum; oxalic acid; amidase; virulence
Genre
Article
Type
Text
Language
eng
Date Available
2024-11-07
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 4.0
DOI
10.14288/1.0447225
URI
http://hdl.handle.net/2429/89619
Affiliation
Science, Faculty of; Non UBC; Botany, Department of
Citation
International Journal of Molecular Sciences 23 (19): 11207 (2022)
Publisher DOI
10.3390/ijms231911207
Peer Review Status
Reviewed
Scholarly Level
Faculty; Researcher
Rights URI
https://creativecommons.org/licenses/by/4.0/
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CC BY 4.0