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An Overview of Pre-Analytical Factors Impacting Metabolomics Analyses of Blood Samples Thachil, Amy; Wang, Li Qing; Mandal, Rupasri; Wishart, David; Blydt-Hansen, Tom

Abstract

Discrepant sample processing remains a significant challenge within blood metabolomics research, introducing non-biological variation into the measured metabolome and biasing downstream results. Inconsistency during the pre-analytical phase can influence experimental processes, producing metabolome measurements that are non-representative of in vivo composition. To minimize variation, there is a need to create and adhere to standardized pre-analytical protocols for blood samples intended for use in metabolomics analyses. This will allow for reliable and reproducible findings within blood metabolomics research. In this review article, we provide an overview of the existing literature pertaining to pre-analytical factors that influence blood metabolite measurements. Pre-analytical factors including blood tube selection, pre- and post-processing time and temperature conditions, centrifugation conditions, freeze–thaw cycles, and long-term storage conditions are specifically discussed, with recommendations provided for best practices at each stage.

Item Metadata

Title
An Overview of Pre-Analytical Factors Impacting Metabolomics Analyses of Blood Samples
Creator
Thachil, Amy; Wang, Li Qing; Mandal, Rupasri; Wishart, David; Blydt-Hansen, Tom
Contributor
Children's Hospital (Vancouver, B.C.). Research Institute; Children's Hospital (Vancouver, B.C.)
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2024-08-28
Description
Discrepant sample processing remains a significant challenge within blood metabolomics research, introducing non-biological variation into the measured metabolome and biasing downstream results. Inconsistency during the pre-analytical phase can influence experimental processes, producing metabolome measurements that are non-representative of in vivo composition. To minimize variation, there is a need to create and adhere to standardized pre-analytical protocols for blood samples intended for use in metabolomics analyses. This will allow for reliable and reproducible findings within blood metabolomics research. In this review article, we provide an overview of the existing literature pertaining to pre-analytical factors that influence blood metabolite measurements. Pre-analytical factors including blood tube selection, pre- and post-processing time and temperature conditions, centrifugation conditions, freeze–thaw cycles, and long-term storage conditions are specifically discussed, with recommendations provided for best practices at each stage.
Subject
metabolomics; blood; sample processing; pre-analytical; protocol
Genre
Article
Type
Text
Language
eng
Date Available
2024-10-04
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 4.0
DOI
10.14288/1.0445494
URI
http://hdl.handle.net/2429/89336
Affiliation
Medicine, Faculty of; Non UBC; Pathology and Laboratory Medicine, Department of; Pediatrics, Department of
Citation
Metabolites 14 (9): 474 (2024)
Publisher DOI
10.3390/metabo14090474
Peer Review Status
Reviewed
Scholarly Level
Faculty; Researcher
Rights URI
https://creativecommons.org/licenses/by/4.0/
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CC BY 4.0