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Green Synthesis of Silver Nanoparticles with Extracts from Kalanchoe fedtschenkoi: Characterization and Bioactivities Mejía-Méndez, Jorge L.; Sánchez-Ante, Gildardo; Cerro-López, Mónica; Minutti-Calva, Yulianna; Navarro-López, Diego E.; Lozada-Ramírez, J. Daniel; Bach, Horacio; López-Mena, Edgar R.; Sánchez-Arreola, Eugenio
Abstract
In this work, the hexane, chloroform, and methanol extracts from Kalanchoe fedtschenkoi were utilized to green-synthesize silver nanoparticles (Kf₁-, Kf₂-, and Kf₃-AgNPs). The Kf₁-, Kf₂-, and Kf₃-AgNPs were characterized by spectroscopy and microscopy techniques. The antibacterial activity of AgNPs was studied against bacteria strains, utilizing the microdilution assay. The DPPH and H₂O₂ assays were considered to assess the antioxidant activity of AgNPs. The results revealed that Kf₁-, Kf₂-, and Kf₃-AgNPs exhibit an average diameter of 39.9, 111, and 42 nm, respectively. The calculated ζ-potential of Kf₁-, Kf₂-, and Kf₃-AgNPs were −20.5, −10.6, and −7.9 mV, respectively. The UV-vis analysis of the three samples demonstrated characteristic absorption bands within the range of 350–450 nm, which confirmed the formation of AgNPs. The FTIR analysis of AgNPs exhibited a series of bands from 3500 to 750 cm-¹, related to the presence of extracts on their surfaces. SEM observations unveiled that Kf₁- and Kf₂-AgNPs adopted structural arrangements related to nano-popcorns and nanoflowers, whereas Kf₃-AgNPs were spherical in shape. It was determined that treatment with Kf₁-, Kf₂-, and Kf₃-AgNPs was demonstrated to inhibit the growth of E. coli, S. aureus, and P. aeruginosa in a dose-dependent manner (50–300 μg/mL). Within the same range, treatment with Kf₁-, Kf₂-, and Kf₃-AgNPs decreased the generation of DPPH (IC₅₀ 57.02–2.09 μg/mL) and H₂O₂ (IC₅₀ 3.15–3.45 μg/mL) radicals. This study highlights the importance of using inorganic nanomaterials to improve the biological performance of plant extracts as an efficient nanotechnological approach.
Item Metadata
| Title |
Green Synthesis of Silver Nanoparticles with Extracts from Kalanchoe fedtschenkoi: Characterization and Bioactivities
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| Creator | |
| Contributor | |
| Publisher |
Multidisciplinary Digital Publishing Institute
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| Date Issued |
2024-06-30
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| Description |
In this work, the hexane, chloroform, and methanol extracts from Kalanchoe fedtschenkoi were utilized to green-synthesize silver nanoparticles (Kf₁-, Kf₂-, and Kf₃-AgNPs). The Kf₁-, Kf₂-, and Kf₃-AgNPs were characterized by spectroscopy and microscopy techniques. The antibacterial activity of AgNPs was studied against bacteria strains, utilizing the microdilution assay. The DPPH and H₂O₂ assays were considered to assess the antioxidant activity of AgNPs. The results revealed that Kf₁-, Kf₂-, and Kf₃-AgNPs exhibit an average diameter of 39.9, 111, and 42 nm, respectively. The calculated ζ-potential of Kf₁-, Kf₂-, and Kf₃-AgNPs were −20.5, −10.6, and −7.9 mV, respectively. The UV-vis analysis of the three samples demonstrated characteristic absorption bands within the range of 350–450 nm, which confirmed the formation of AgNPs. The FTIR analysis of AgNPs exhibited a series of bands from 3500 to 750 cm-¹, related to the presence of extracts on their surfaces. SEM observations unveiled that Kf₁- and Kf₂-AgNPs adopted structural arrangements related to nano-popcorns and nanoflowers, whereas Kf₃-AgNPs were spherical in shape. It was determined that treatment with Kf₁-, Kf₂-, and Kf₃-AgNPs was demonstrated to inhibit the growth of E. coli, S. aureus, and P. aeruginosa in a dose-dependent manner (50–300 μg/mL). Within the same range, treatment with Kf₁-, Kf₂-, and Kf₃-AgNPs decreased the generation of DPPH (IC₅₀ 57.02–2.09 μg/mL) and H₂O₂ (IC₅₀ 3.15–3.45 μg/mL) radicals. This study highlights the importance of using inorganic nanomaterials to improve the biological performance of plant extracts as an efficient nanotechnological approach.
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| Subject | |
| Genre | |
| Type | |
| Language |
eng
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| Date Available |
2024-08-02
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| Provider |
Vancouver : University of British Columbia Library
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| Rights |
CC BY 4.0
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| DOI |
10.14288/1.0444972
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| URI | |
| Affiliation | |
| Citation |
Biomolecules 14 (7): 782 (2024)
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| Publisher DOI |
10.3390/biom14070782
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| Peer Review Status |
Reviewed
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| Scholarly Level |
Faculty; Researcher
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| Rights URI | |
| Aggregated Source Repository |
DSpace
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CC BY 4.0