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Precise Serial Microregistration Enables Quantitative Microscopy Imaging Tracking of Human Skin Cells In Vivo Tian, Yunxian; Wu, Zhenguo; Lui, Harvey; Zhao, Jianhua; Kalia, Sunil; Seo, InSeok; Ou-Yang, Hao; Zeng, Haishan

Abstract

We developed an automated microregistration method that enables repeated in vivo skin microscopy imaging of the same tissue microlocation and specific cells over a long period of days and weeks with unprecedented precision. Applying this method in conjunction with an in vivo multimodality multiphoton microscope, the behavior of human skin cells such as cell proliferation, melanin upward migration, blood flow dynamics, and epidermal thickness adaptation can be recorded over time, facilitating quantitative cellular dynamics analysis. We demonstrated the usefulness of this method in a skin biology study by successfully monitoring skin cellular responses for a period of two weeks following an acute exposure to ultraviolet light.

Item Metadata

Title
Precise Serial Microregistration Enables Quantitative Microscopy Imaging Tracking of Human Skin Cells In Vivo
Creator
Tian, Yunxian; Wu, Zhenguo; Lui, Harvey; Zhao, Jianhua; Kalia, Sunil; Seo, InSeok; Ou-Yang, Hao; Zeng, Haishan
Contributor
BC Cancer Research Centre. Integrative Oncology; Vancouver Coastal Health Authority. Research Institute
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2024-07-07
Description
We developed an automated microregistration method that enables repeated in vivo skin microscopy imaging of the same tissue microlocation and specific cells over a long period of days and weeks with unprecedented precision. Applying this method in conjunction with an in vivo multimodality multiphoton microscope, the behavior of human skin cells such as cell proliferation, melanin upward migration, blood flow dynamics, and epidermal thickness adaptation can be recorded over time, facilitating quantitative cellular dynamics analysis. We demonstrated the usefulness of this method in a skin biology study by successfully monitoring skin cellular responses for a period of two weeks following an acute exposure to ultraviolet light.
Subject
in vivo microscopy; reflectance confocal microscopy; multiphoton microscopy; two-photon excitation fluorescence; second harmonic generation; in vivo cellular imaging; in vivo cell tracking; human skin; melanin; epidermal; UV radiation; longitudinal study
Genre
Article
Type
Text
Language
eng
Date Available
2024-07-30
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 4.0
DOI
10.14288/1.0444855
URI
http://hdl.handle.net/2429/88817
Affiliation
Medicine, Faculty of; Non UBC; Dermatology and Skin Science, Department of
Citation
Cells 13 (13): 1158 (2024)
Publisher DOI
10.3390/cells13131158
Peer Review Status
Reviewed
Scholarly Level
Faculty; Researcher
Rights URI
https://creativecommons.org/licenses/by/4.0/
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CC BY 4.0