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SsCak1 Regulates Growth and Pathogenicity in Sclerotinia sclerotiorum Qin, Lei; Nong, Jieying; Cui, Kan; Tang, Xianyu; Gong, Xin; Xia, Yunong; Xu, Yan; Qiu, Yilan; Li, Xin; Xia, Shitou

Abstract

Sclerotinia sclerotiorum is a devastating fungal pathogen that causes severe crop losses worldwide. It is of vital importance to understand its pathogenic mechanism for disease control. Through a forward genetic screen combined with next-generation sequencing, a putative protein kinase, SsCak1, was found to be involved in the growth and pathogenicity of S. sclerotiorum. Knockout and complementation experiments confirmed that deletions in SsCak1 caused defects in mycelium and sclerotia development, as well as appressoria formation and host penetration, leading to complete loss of virulence. These findings suggest that SsCak1 is essential for the growth, development, and pathogenicity of S. sclerotiorum. Therefore, SsCak1 could serve as a potential target for the control of S. sclerotiorum infection through host-induced gene silencing (HIGS), which could increase crop resistance to the pathogen.

Item Metadata

Title
SsCak1 Regulates Growth and Pathogenicity in Sclerotinia sclerotiorum
Creator
Qin, Lei; Nong, Jieying; Cui, Kan; Tang, Xianyu; Gong, Xin; Xia, Yunong; Xu, Yan; Qiu, Yilan; Li, Xin; Xia, Shitou
Contributor
Michael Smith Laboratories
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2023-08-09
Description
Sclerotinia sclerotiorum is a devastating fungal pathogen that causes severe crop losses worldwide. It is of vital importance to understand its pathogenic mechanism for disease control. Through a forward genetic screen combined with next-generation sequencing, a putative protein kinase, SsCak1, was found to be involved in the growth and pathogenicity of S. sclerotiorum. Knockout and complementation experiments confirmed that deletions in SsCak1 caused defects in mycelium and sclerotia development, as well as appressoria formation and host penetration, leading to complete loss of virulence. These findings suggest that SsCak1 is essential for the growth, development, and pathogenicity of S. sclerotiorum. Therefore, SsCak1 could serve as a potential target for the control of S. sclerotiorum infection through host-induced gene silencing (HIGS), which could increase crop resistance to the pathogen.
Subject
Sclerotinia sclerotiorum; SsCak1; sclerotia; appressoria; pathogenesis; HIGS
Genre
Article
Type
Text
Language
eng
Date Available
2023-09-28
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 4.0
DOI
10.14288/1.0436911
URI
http://hdl.handle.net/2429/86048
Affiliation
Science, Faculty of; Non UBC; Botany, Department of
Citation
International Journal of Molecular Sciences 24 (16): 12610 (2023)
Publisher DOI
10.3390/ijms241612610
Peer Review Status
Reviewed
Scholarly Level
Faculty; Researcher
Rights URI
https://creativecommons.org/licenses/by/4.0/
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CC BY 4.0