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Microscopic marine invertebrates are reservoirs for cryptic and diverse protists and fungi Holt, Corey C.; Boscaro, Vittorio; Van Steenkiste, Niels W. L.; Herranz, Maria; Mathur, Varsha; Irwin, Nicholas A. T.; Buckholtz, Gracy; Leander, Brian S.; Keeling, Patrick J. (Patrick John), 1969-

Abstract

Background: Microbial symbioses in marine invertebrates are commonplace. However, characterizations of invertebrate microbiomes are vastly outnumbered by those of vertebrates. Protists and fungi run the gamut of symbiosis, yet eukaryotic microbiome sequencing is rarely undertaken, with much of the focus on bacteria. To explore the importance of microscopic marine invertebrates as potential symbiont reservoirs, we used a phylogenetic-focused approach to analyze the host-associated eukaryotic microbiomes of 220 animal specimens spanning nine different animal phyla. Results Our data expanded the traditional host range of several microbial taxa and identified numerous undescribed lineages. A lack of comparable reference sequences resulted in several cryptic clades within the Apicomplexa and Ciliophora and emphasized the potential for microbial invertebrates to harbor novel protistan and fungal diversity. Conclusions Microscopic marine invertebrates, spanning a wide range of animal phyla, host various protist and fungal sequences and may therefore serve as a useful resource in the detection and characterization of undescribed symbioses. Video Abstract

Item Metadata

Title
Microscopic marine invertebrates are reservoirs for cryptic and diverse protists and fungi
Creator
Holt, Corey C.; Boscaro, Vittorio; Van Steenkiste, Niels W. L.; Herranz, Maria; Mathur, Varsha; Irwin, Nicholas A. T.; Buckholtz, Gracy; Leander, Brian S.; Keeling, Patrick J. (Patrick John), 1969-
Publisher
BioMed Central
Date Issued
2022-09-30
Description
Background: Microbial symbioses in marine invertebrates are commonplace. However, characterizations of invertebrate microbiomes are vastly outnumbered by those of vertebrates. Protists and fungi run the gamut of symbiosis, yet eukaryotic microbiome sequencing is rarely undertaken, with much of the focus on bacteria. To explore the importance of microscopic marine invertebrates as potential symbiont reservoirs, we used a phylogenetic-focused approach to analyze the host-associated eukaryotic microbiomes of 220 animal specimens spanning nine different animal phyla. Results Our data expanded the traditional host range of several microbial taxa and identified numerous undescribed lineages. A lack of comparable reference sequences resulted in several cryptic clades within the Apicomplexa and Ciliophora and emphasized the potential for microbial invertebrates to harbor novel protistan and fungal diversity. Conclusions Microscopic marine invertebrates, spanning a wide range of animal phyla, host various protist and fungal sequences and may therefore serve as a useful resource in the detection and characterization of undescribed symbioses. Video Abstract
Subject
Aquatic; Invertebrate; Microbiota; 18S; ASV; Symbiont; Host-associated
Genre
Article
Type
Text
Language
eng
Date Available
2023-09-26
Provider
Vancouver : University of British Columbia Library
Rights
Attribution 4.0 International (CC BY 4.0)
DOI
10.14288/1.0436868
URI
http://hdl.handle.net/2429/86008
Affiliation
Science, Faculty of; Non UBC; Botany, Department of; Zoology, Department of
Citation
Holt, C.C., Boscaro, V., Van Steenkiste, N.W.L. et al. Microscopic marine invertebrates are reservoirs for cryptic and diverse protists and fungi. Microbiome 10, 161 (2022).
Publisher DOI
10.1186/s40168-022-01363-3
Peer Review Status
Reviewed
Scholarly Level
Faculty; Researcher; Postdoctoral; Graduate
Copyright Holder
The Author(s)
Rights URI
http://creativecommons.org/licenses/by/4.0/
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Attribution 4.0 International (CC BY 4.0)