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Relevance of Host Cell Surface Glycan Structure for Cell Specificity of Influenza A Viruses Kastner, Markus; Karner, Andreas; Zhu, Rong; Huang, Qiang; Geissner, Andreas; Sadewasser, Anne; Lesch, Markus; Wörmann, Xenia; Karlas, Alexander; Seeberger, Peter H.; Wolff, Thorsten; Hinterdorfer, Peter; Herrmann, Andreas; Sieben, Christian

Abstract

Influenza A viruses (IAVs) initiate infection via binding of the viral hemagglutinin (HA) to sialylated glycans on host cells. HA’s receptor specificity towards individual glycans is well studied and clearly critical for virus infection, but the contribution of the highly heterogeneous and complex glycocalyx to virus–cell adhesion remains elusive. Here, we use two complementary methods, glycan arrays and single-virus force spectroscopy (SVFS), to compare influenza virus receptor specificity with virus binding to live cells. Unexpectedly, we found that HA’s receptor binding preference does not necessarily reflect virus–cell specificity. We propose SVFS as a tool to elucidate the cell binding preference of IAVs, thereby including the complex environment of sialylated receptors within the plasma membrane of living cells.

Item Metadata

Title
Relevance of Host Cell Surface Glycan Structure for Cell Specificity of Influenza A Viruses
Creator
Kastner, Markus; Karner, Andreas; Zhu, Rong; Huang, Qiang; Geissner, Andreas; Sadewasser, Anne; Lesch, Markus; Wörmann, Xenia; Karlas, Alexander; Seeberger, Peter H.; Wolff, Thorsten; Hinterdorfer, Peter; Herrmann, Andreas; Sieben, Christian
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2023-07-05
Description
Influenza A viruses (IAVs) initiate infection via binding of the viral hemagglutinin (HA) to sialylated glycans on host cells. HA’s receptor specificity towards individual glycans is well studied and clearly critical for virus infection, but the contribution of the highly heterogeneous and complex glycocalyx to virus–cell adhesion remains elusive. Here, we use two complementary methods, glycan arrays and single-virus force spectroscopy (SVFS), to compare influenza virus receptor specificity with virus binding to live cells. Unexpectedly, we found that HA’s receptor binding preference does not necessarily reflect virus–cell specificity. We propose SVFS as a tool to elucidate the cell binding preference of IAVs, thereby including the complex environment of sialylated receptors within the plasma membrane of living cells.
Subject
influenza A virus; cell binding; force spectroscopy
Genre
Article
Type
Text
Language
eng
Date Available
2023-08-01
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 4.0
DOI
10.14288/1.0434636
URI
http://hdl.handle.net/2429/85358
Affiliation
Science, Faculty of; Non UBC; Chemistry, Department of
Citation
Viruses 15 (7): 1507 (2023)
Publisher DOI
10.3390/v15071507
Peer Review Status
Reviewed
Scholarly Level
Faculty; Researcher
Rights URI
https://creativecommons.org/licenses/by/4.0/
Aggregated Source Repository
DSpace

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CC BY 4.0