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Relevance of Host Cell Surface Glycan Structure for Cell Specificity of Influenza A Viruses Kastner, Markus; Karner, Andreas; Zhu, Rong; Huang, Qiang; Geissner, Andreas; Sadewasser, Anne; Lesch, Markus; Wörmann, Xenia; Karlas, Alexander; Seeberger, Peter H.; Wolff, Thorsten; Hinterdorfer, Peter; Herrmann, Andreas; Sieben, Christian
Abstract
Influenza A viruses (IAVs) initiate infection via binding of the viral hemagglutinin (HA) to sialylated glycans on host cells. HA’s receptor specificity towards individual glycans is well studied and clearly critical for virus infection, but the contribution of the highly heterogeneous and complex glycocalyx to virus–cell adhesion remains elusive. Here, we use two complementary methods, glycan arrays and single-virus force spectroscopy (SVFS), to compare influenza virus receptor specificity with virus binding to live cells. Unexpectedly, we found that HA’s receptor binding preference does not necessarily reflect virus–cell specificity. We propose SVFS as a tool to elucidate the cell binding preference of IAVs, thereby including the complex environment of sialylated receptors within the plasma membrane of living cells.
Item Metadata
Title |
Relevance of Host Cell Surface Glycan Structure for Cell Specificity of Influenza A Viruses
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Creator | |
Publisher |
Multidisciplinary Digital Publishing Institute
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Date Issued |
2023-07-05
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Description |
Influenza A viruses (IAVs) initiate infection via binding of the viral hemagglutinin (HA) to sialylated glycans on host cells. HA’s receptor specificity towards individual glycans is well studied and clearly critical for virus infection, but the contribution of the highly heterogeneous and complex glycocalyx to virus–cell adhesion remains elusive. Here, we use two complementary methods, glycan arrays and single-virus force spectroscopy (SVFS), to compare influenza virus receptor specificity with virus binding to live cells. Unexpectedly, we found that HA’s receptor binding preference does not necessarily reflect virus–cell specificity. We propose SVFS as a tool to elucidate the cell binding preference of IAVs, thereby including the complex environment of sialylated receptors within the plasma membrane of living cells.
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Subject | |
Genre | |
Type | |
Language |
eng
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Date Available |
2023-08-01
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Provider |
Vancouver : University of British Columbia Library
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Rights |
CC BY 4.0
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DOI |
10.14288/1.0434636
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URI | |
Affiliation | |
Citation |
Viruses 15 (7): 1507 (2023)
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Publisher DOI |
10.3390/v15071507
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Peer Review Status |
Reviewed
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Scholarly Level |
Faculty; Researcher
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Rights URI | |
Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
CC BY 4.0