Two-Dimensional Interfacial Exchange Diffusion Has the Potential to Augment Spatiotemporal Precision of Ca²⁺ Signaling

UBC Faculty Research and Publications

Two-Dimensional Interfacial Exchange Diffusion Has the Potential to Augment Spatiotemporal Precision of Ca²⁺ Signaling van Breemen, Cornelis; Fameli, Nicola; Groschner, Klaus; van

Abstract

Nano-junctions between the endoplasmic reticulum and cytoplasmic surfaces of the plasma membrane and other organelles shape the spatiotemporal features of biological Ca²⁺ signals. Herein, we propose that 2D Ca²⁺ exchange diffusion on the negatively charged phospholipid surface lining nano-junctions participates in guiding Ca²⁺ from its source (channel or carrier) to its target (transport protein or enzyme). Evidence provided by in vitro Ca²⁺ flux experiments using an artificial phospholipid membrane is presented in support of the above proposed concept, and results from stochastic simulations of Ca²⁺ trajectories within nano-junctions are discussed in order to substantiate its possible requirements. Finally, we analyze recent literature on Ca²⁺ lipid interactions, which suggests that 2D interfacial Ca²⁺ diffusion may represent an important mechanism of signal transduction in biological systems characterized by high phospholipid surface to aqueous volume ratios.

Item Metadata

Title	Two-Dimensional Interfacial Exchange Diffusion Has the Potential to Augment Spatiotemporal Precision of Ca²⁺ Signaling
Creator	van Breemen, Cornelis; Fameli, Nicola; Groschner, Klaus; van
Publisher	Multidisciplinary Digital Publishing Institute
Date Issued	2022-01-13
Description	Nano-junctions between the endoplasmic reticulum and cytoplasmic surfaces of the plasma membrane and other organelles shape the spatiotemporal features of biological Ca²⁺ signals. Herein, we propose that 2D Ca²⁺ exchange diffusion on the negatively charged phospholipid surface lining nano-junctions participates in guiding Ca²⁺ from its source (channel or carrier) to its target (transport protein or enzyme). Evidence provided by in vitro Ca²⁺ flux experiments using an artificial phospholipid membrane is presented in support of the above proposed concept, and results from stochastic simulations of Ca²⁺ trajectories within nano-junctions are discussed in order to substantiate its possible requirements. Finally, we analyze recent literature on Ca²⁺ lipid interactions, which suggests that 2D interfacial Ca²⁺ diffusion may represent an important mechanism of signal transduction in biological systems characterized by high phospholipid surface to aqueous volume ratios.
Subject	Ca²⁺ signaling; nano-junctions; intermembrane nano-spaces; Ca²⁺ transport; Ca²⁺ lipid interaction; surface diffusion
Genre	Article
Type	Text
Language	eng
Date Available	2022-02-18
Provider	Vancouver : University of British Columbia Library
Rights	CC BY 4.0
DOI	10.14288/1.0406619
URI	http://hdl.handle.net/2429/80857
Affiliation	Medicine, Faculty of; Non UBC; Anesthesiology, Pharmacology and Therapeutics, Department of
Citation	International Journal of Molecular Sciences 23 (2): 850 (2022)
Publisher DOI	10.3390/ijms23020850
Peer Review Status	Reviewed
Scholarly Level	Faculty; Researcher; Other
Rights URI	https://creativecommons.org/licenses/by/4.0/
Aggregated Source Repository	DSpace

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