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Identification of Shiga-Toxin-Producing Shigella Infections in Travel and Non-Travel Related Cases in Alberta, Canada Zhi, Shuai; Parsons, Brendon D.; Szelewicki, Jonas; Yuen, Yue T. K.; Fach, Patrick; Delannoy, Sabine; Li, Vincent; Ferrato, Christina; Freedman, Stephen B.; Lee, Bonita E.; et al.

Abstract

It has long been accepted that Shiga toxin (Stx) only exists in Shigella dysenteriae serotype 1. However, in recent decades, the presence of Shiga toxin genes (stx) in other Shigella spp. have been reported. We screened 366 Shigella flexneri strains from Alberta, Canada (2003 to 2016) for stx and 26 positive strains were identified. These isolates are highly related with the majority originating from the Dominican Republic and three isolates with Haiti origin. Both phylogenetic and spanning tree analysis of the 26 Alberta and 29 stx positive S. flexneri originating from the U.S., France, Canada (Quebec) and Haiti suggests that there are geographic specific distribution patterns (Haiti and Dominican Republic clades). This study provides the first comprehensive whole genome based phylogenetic analysis of stx positive S. flexneri strains as well as their global transmission, which signify the public health risks of global spreading of these strains.

Item Metadata

Title
Identification of Shiga-Toxin-Producing Shigella Infections in Travel and Non-Travel Related Cases in Alberta, Canada
Creator
Zhi, Shuai; Parsons, Brendon D.; Szelewicki, Jonas; Yuen, Yue T. K.; Fach, Patrick; Delannoy, Sabine; Li, Vincent; Ferrato, Christina; Freedman, Stephen B.; Lee, Bonita E.; Pang, Xiao-Li; Chui, Linda
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2021-10-25
Description
It has long been accepted that Shiga toxin (Stx) only exists in Shigella dysenteriae serotype 1. However, in recent decades, the presence of Shiga toxin genes (stx) in other Shigella spp. have been reported. We screened 366 Shigella flexneri strains from Alberta, Canada (2003 to 2016) for stx and 26 positive strains were identified. These isolates are highly related with the majority originating from the Dominican Republic and three isolates with Haiti origin. Both phylogenetic and spanning tree analysis of the 26 Alberta and 29 stx positive S. flexneri originating from the U.S., France, Canada (Quebec) and Haiti suggests that there are geographic specific distribution patterns (Haiti and Dominican Republic clades). This study provides the first comprehensive whole genome based phylogenetic analysis of stx positive S. flexneri strains as well as their global transmission, which signify the public health risks of global spreading of these strains.
Subject
Shigella flexneri; Shiga toxin; phage; phylogenomic; global transmissions
Genre
Article
Type
Text
Language
eng
Date Available
2021-11-29
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 4.0
DOI
10.14288/1.0404393
URI
http://hdl.handle.net/2429/80282
Affiliation
Other UBC; Alumni
Citation
Toxins 13 (11): 755 (2021)
Publisher DOI
10.3390/toxins13110755
Peer Review Status
Reviewed
Scholarly Level
Faculty; Researcher; Other
Rights URI
https://creativecommons.org/licenses/by/4.0/
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Rights

CC BY 4.0