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Characterization of Adaptive-like γδ T Cells in Ugandan Infants during Primary Cytomegalovirus Infection Tuengel, Jessica; Ranchal, Sanya; Maslova, Alexandra; Aulakh, Gurpreet; Papadopoulou, Maria; Drissler, Sibyl; Cai, Bing; Mohsenzadeh-Green, Cetare; Soudeyns, Hugo; Mostafavi, Sara; et al.
Abstract
Gamma-delta (γδ) T cells are unconventional T cells that help control cytomegalovirus (CMV) infection in adults. γδ T cells develop early in gestation, and a fetal public γδ T cell receptor (TCR) clonotype is detected in congenital CMV infections. However, age-dependent γδ T cell responses to primary CMV infection are not well-understood. Flow cytometry and TCR sequencing was used to comprehensively characterize γδ T cell responses to CMV infection in a cohort of 32 infants followed prospectively from birth. Peripheral blood γδ T cell frequencies increased during infancy, and were higher among CMV-infected infants relative to uninfected. Clustering analyses revealed associations between CMV infection and activation marker expression on adaptive-like Vδ1 and Vδ3, but not innate-like Vγ9Vδ2 γδ T cell subsets. Frequencies of NKG2C⁺CD57⁺ γδ T cells were temporally associated with the quantity of CMV shed in saliva by infants with primary infection. The public γδ TCR clonotype was only detected in CMV-infected infants <120 days old and at lower frequencies than previously described in fetal infections. Our findings support the notion that CMV infection drives age-dependent expansions of specific γδ T cell populations, and provide insight for novel strategies to prevent CMV transmission and disease.
Item Metadata
Title |
Characterization of Adaptive-like γδ T Cells in Ugandan Infants during Primary Cytomegalovirus Infection
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Creator | |
Contributor | |
Publisher |
Multidisciplinary Digital Publishing Institute
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Date Issued |
2021-10-03
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Description |
Gamma-delta (γδ) T cells are unconventional T cells that help control cytomegalovirus
(CMV) infection in adults. γδ T cells develop early in gestation, and a fetal public γδ T cell receptor
(TCR) clonotype is detected in congenital CMV infections. However, age-dependent γδ T cell
responses to primary CMV infection are not well-understood. Flow cytometry and TCR sequencing
was used to comprehensively characterize γδ T cell responses to CMV infection in a cohort of
32 infants followed prospectively from birth. Peripheral blood γδ T cell frequencies increased during
infancy, and were higher among CMV-infected infants relative to uninfected. Clustering analyses
revealed associations between CMV infection and activation marker expression on adaptive-like Vδ1
and Vδ3, but not innate-like Vγ9Vδ2 γδ T cell subsets. Frequencies of NKG2C⁺CD57⁺ γδ T cells were
temporally associated with the quantity of CMV shed in saliva by infants with primary infection.
The public γδ TCR clonotype was only detected in CMV-infected infants <120 days old and at lower
frequencies than previously described in fetal infections. Our findings support the notion that CMV
infection drives age-dependent expansions of specific γδ T cell populations, and provide insight for
novel strategies to prevent CMV transmission and disease.
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Subject | |
Genre | |
Type | |
Language |
eng
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Date Available |
2021-11-25
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Provider |
Vancouver : University of British Columbia Library
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Rights |
CC BY 4.0
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DOI |
10.14288/1.0403827
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URI | |
Affiliation | |
Citation |
Viruses 13 (10): 1987 (2021)
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Publisher DOI |
10.3390/v13101987
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Peer Review Status |
Reviewed
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Scholarly Level |
Faculty; Researcher
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Rights URI | |
Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
CC BY 4.0