- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Faculty Research and Publications /
- Inhaled Nitric Oxide Therapy Fails to Improve Outcome...
Open Collections
UBC Faculty Research and Publications
Inhaled Nitric Oxide Therapy Fails to Improve Outcome in Experimental Severe Influenza Darwish, Ilyse; Miller, Chris; Kain, Kevin C.; Liles, W. Conrad
Abstract
In vitro, nitric oxide (NO) has been shown to have antimicrobial activity against a wide range of viruses, including influenza A virus. Therefore, we hypothesized that inhaled nitric oxide (iNO) would increase survival in vivo by reducing the viral load in C57Bl/6 mice infected with a lethal dose of influenza A/WSN/33 (H1N1; WSN/33) virus. NO was delivered to influenza-infected mice either continuously or intermittently at 80 or 160 ppm, respectively, using both prophylactic and post-infection treatment strategies. Murine survival and weight loss were assessed, and lung viral load was quantified via plaque assay. Here, we report that iNO administered prophylactically or post-influenza infection failed to improve survival of infected mice. No difference in lung viral load was observed between experimental groups. Although NO has antiviral activity against influenza A virus in vitro, iNO therapy provided no apparent benefit when used for treatment of influenza A virus infection in vivo.
Item Metadata
Title |
Inhaled Nitric Oxide Therapy Fails to Improve Outcome in Experimental Severe Influenza
|
Creator | |
Publisher |
Ivyspring International Publisher
|
Date Issued |
2012-01-13
|
Description |
In vitro, nitric oxide (NO) has been shown to have antimicrobial activity against a wide range of
viruses, including influenza A virus. Therefore, we hypothesized that inhaled nitric oxide
(iNO) would increase survival in vivo by reducing the viral load in C57Bl/6 mice infected with
a lethal dose of influenza A/WSN/33 (H1N1; WSN/33) virus. NO was delivered to influenza-infected mice either continuously or intermittently at 80 or 160 ppm, respectively, using
both prophylactic and post-infection treatment strategies. Murine survival and weight loss
were assessed, and lung viral load was quantified via plaque assay. Here, we report that iNO
administered prophylactically or post-influenza infection failed to improve survival of infected
mice. No difference in lung viral load was observed between experimental groups. Although
NO has antiviral activity against influenza A virus in vitro, iNO therapy provided no apparent
benefit when used for treatment of influenza A virus infection in vivo.
|
Subject | |
Genre | |
Type | |
Language |
eng
|
Date Available |
2021-06-08
|
Provider |
Vancouver : University of British Columbia Library
|
Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
|
DOI |
10.14288/1.0398279
|
URI | |
Affiliation | |
Citation |
Darwish I, Miller C, Kain KC, Liles WC. Inhaled Nitric Oxide Therapy Fails to Improve Outcome in Experimental Severe Influenza. Int J Med Sci 2012; 9(2):157-162.
|
Publisher DOI |
10.7150/ijms.3880
|
Peer Review Status |
Reviewed
|
Scholarly Level |
Faculty; Graduate
|
Copyright Holder |
Ivyspring International Publisher
|
Rights URI | |
Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International