Open Collections

UBC Faculty Research and Publications

Genome wide association study of response to interval and continuous exercise training: the Predict-HIIT study Williams, Camilla J; Li, Zhixiu; Harvey, Nicholas; Lea, Rodney A; Gurd, Brendon J; Bonafiglia, Jacob T; Papadimitriou, Ioannis; Jacques, Macsue; Croci, Ilaria; Stensvold, Dorthe; et al.

Abstract

Background: Low cardiorespiratory fitness (V̇O₂peak) is highly associated with chronic disease and mortality from all causes. Whilst exercise training is recommended in health guidelines to improve V̇O2peak, there is considerable inter-individual variability in the V̇O₂peak response to the same dose of exercise. Understanding how genetic factors contribute to V̇O₂peak training response may improve personalisation of exercise programs. The aim of this study was to identify genetic variants that are associated with the magnitude of V̇O₂peak response following exercise training. Methods: Participant change in objectively measured V̇O₂peak from 18 different interventions was obtained from a multi-centre study (Predict-HIIT). A genome-wide association study was completed (n = 507), and a polygenic predictor score (PPS) was developed using alleles from single nucleotide polymorphisms (SNPs) significantly associated (P < 1 × 10⁻⁵) with the magnitude of V̇O₂peak response. Findings were tested in an independent validation study (n = 39) and compared to previous research. Results: No variants at the genome-wide significance level were found after adjusting for key covariates (baseline V̇O₂peak, individual study, principal components which were significantly associated with the trait). A Quantile–Quantile plot indicates there was minor inflation in the study. Twelve novel loci showed a trend of association with V̇O₂peak response that reached suggestive significance (P < 1 × 10⁻⁵). The strongest association was found near the membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2) gene (rs6959961, P = 2.61 × 10⁻⁷). A PPS created from the 12 lead SNPs was unable to predict V̇O2peak response in a tenfold cross validation, or in an independent (n = 39) validation study (P > 0.1). Significant correlations were found for beta coefficients of variants in the Predict-HIIT (P < 1 × 10⁻⁴) and the validation study (P <  × 10⁻⁶), indicating that general effects of the loci exist, and that with a higher statistical power, more significant genetic associations may become apparent. Conclusions: Ongoing research and validation of current and previous findings is needed to determine if genetics does play a large role in V̇O₂peak response variance, and whether genomic predictors for V̇O2peak response trainability can inform evidence-based clinical practice. Trial registration Australian New Zealand Clinical Trials Registry (ANZCTR), Trial Id: ACTRN12618000501246, Date Registered: 06/04/2018, http://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=374601&isReview=true .

Item Metadata

Title
Genome wide association study of response to interval and continuous exercise training: the Predict-HIIT study
Creator
Williams, Camilla J; Li, Zhixiu; Harvey, Nicholas; Lea, Rodney A; Gurd, Brendon J; Bonafiglia, Jacob T; Papadimitriou, Ioannis; Jacques, Macsue; Croci, Ilaria; Stensvold, Dorthe; Wisloff, Ulrik; Taylor, Jenna L; Gajanand, Trishan; Cox, Emily R; Ramos, Joyce S; Fassett, Robert G; Little, Jonathan P.; Francois, Monique E.; Hearon, Christopher M; Sarma, Satyam; Janssen, Sylvan L J E; Van Craenenbroeck, Emeline M; Beckers, Paul; Cornelissen, Véronique A; Howden, Erin J; Keating, Shelley E; Yan, Xu; Bishop, David J; Bye, Anja; Haupt, Larisa M; Griffiths, Lyn R; Ashton, Kevin J; Brown, Matthew A; Torquati, Luciana; Eynon, Nir; Coombes, Jeff S
Publisher
BioMed Central
Date Issued
2021-05-13
Description
Background: Low cardiorespiratory fitness (V̇O₂peak) is highly associated with chronic disease and mortality from all causes. Whilst exercise training is recommended in health guidelines to improve V̇O2peak, there is considerable inter-individual variability in the V̇O₂peak response to the same dose of exercise. Understanding how genetic factors contribute to V̇O₂peak training response may improve personalisation of exercise programs. The aim of this study was to identify genetic variants that are associated with the magnitude of V̇O₂peak response following exercise training. Methods: Participant change in objectively measured V̇O₂peak from 18 different interventions was obtained from a multi-centre study (Predict-HIIT). A genome-wide association study was completed (n = 507), and a polygenic predictor score (PPS) was developed using alleles from single nucleotide polymorphisms (SNPs) significantly associated (P < 1 × 10⁻⁵) with the magnitude of V̇O₂peak response. Findings were tested in an independent validation study (n = 39) and compared to previous research. Results: No variants at the genome-wide significance level were found after adjusting for key covariates (baseline V̇O₂peak, individual study, principal components which were significantly associated with the trait). A Quantile–Quantile plot indicates there was minor inflation in the study. Twelve novel loci showed a trend of association with V̇O₂peak response that reached suggestive significance (P < 1 × 10⁻⁵). The strongest association was found near the membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2) gene (rs6959961, P = 2.61 × 10⁻⁷). A PPS created from the 12 lead SNPs was unable to predict V̇O2peak response in a tenfold cross validation, or in an independent (n = 39) validation study (P > 0.1). Significant correlations were found for beta coefficients of variants in the Predict-HIIT (P < 1 × 10⁻⁴) and the validation study (P <  × 10⁻⁶), indicating that general effects of the loci exist, and that with a higher statistical power, more significant genetic associations may become apparent. Conclusions: Ongoing research and validation of current and previous findings is needed to determine if genetics does play a large role in V̇O₂peak response variance, and whether genomic predictors for V̇O2peak response trainability can inform evidence-based clinical practice. Trial registration Australian New Zealand Clinical Trials Registry (ANZCTR), Trial Id: ACTRN12618000501246, Date Registered: 06/04/2018, http://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=374601&isReview=true .
Subject
Genetics; V̇O2peak training response; Individual variability; GWAS; Polygenic predictor score
Genre
Article
Type
Text
Language
eng
Date Available
2021-05-13
Provider
Vancouver : University of British Columbia Library
Rights
Attribution 4.0 International (CC BY 4.0)
DOI
10.14288/1.0397480
URI
http://hdl.handle.net/2429/78333
Affiliation
Health and Social Development, Faculty of (Okanagan); Non UBC; Health and Exercise Sciences, School of (Okanagan)
Citation
Journal of Biomedical Science. 2021 May 13;28(1):37
Publisher DOI
10.1186/s12929-021-00733-7
Peer Review Status
Reviewed
Scholarly Level
Faculty
Copyright Holder
The Author(s)
Rights URI
http://creativecommons.org/licenses/by/4.0/
Aggregated Source Repository
DSpace

Item Media

Item Citations and Data

Rights

Attribution 4.0 International (CC BY 4.0)