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A novel, well-resolved direct laser bioprinting system for rapid cell encapsulation and microwell fabrication Wang, Zongjie; Jin, Xian; Tian, Zhenlin; Menard, Frederic; Holzman, Jonathan; Kim, Keekyoung

Abstract

A direct laser bioprinting (DLBP) system is introduced in this work. The DLBP system applies visible-laser-induced photocrosslinking at a wavelength of 405 nm using the photoinitiator VA086. It is shown that such a system can fabricate vertical structures with fine features (less than 50 μm), and high cell viability (greater than 95%). Experimental characterizations and theoretical simulations are presented, with good agreement seen between the experiments and theory. The DLBP system is applied to the fabrication of (1) cell-laden hydrogel microgrids, (2) hydrogel microwells, as well as a test of (3) cell encapsulation, and (4) cell seeding. The DLBP system is found to be a promising tool for bioprinting.

Item Metadata

Title
A novel, well-resolved direct laser bioprinting system for rapid cell encapsulation and microwell fabrication
Creator
Wang, Zongjie; Jin, Xian; Tian, Zhenlin; Menard, Frederic; Holzman, Jonathan; Kim, Keekyoung
Publisher
Wiley
Date Issued
2018-02-05
Description
A direct laser bioprinting (DLBP) system is introduced in this work. The DLBP system applies visible-laser-induced photocrosslinking at a wavelength of 405 nm using the photoinitiator VA086. It is shown that such a system can fabricate vertical structures with fine features (less than 50 μm), and high cell viability (greater than 95%). Experimental characterizations and theoretical simulations are presented, with good agreement seen between the experiments and theory. The DLBP system is applied to the fabrication of (1) cell-laden hydrogel microgrids, (2) hydrogel microwells, as well as a test of (3) cell encapsulation, and (4) cell seeding. The DLBP system is found to be a promising tool for bioprinting.
Subject
laser bioprinting; rapid cell encapsulation; biofabrication; microwells; tissue engineering; poly(ethylene glycol)
Genre
Article
Type
Text
Language
eng
Date Available
2021-04-09
Provider
Vancouver : University of British Columbia Library
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International
DOI
10.14288/1.0396650
URI
http://hdl.handle.net/2429/77734
Affiliation
Applied Science, Faculty of; Science, Irving K. Barber Faculty of (Okanagan); Biomedical Engineering, School of; Chemistry, Department of (Okanagan); Engineering, School of (Okanagan)
Citation
Z. Wang, X. Jin, Z. Tian, F. Menard, J. F. Holzman, and K. Kim, "A novel, well-resolved direct laser bioprinting system for rapid cell encapsulation and microwell fabrication," Advanced Healthcare Materials, vol. 7, pp. 1701249(1-11), 2018
Publisher DOI
10.1002/adhm.201701249
Peer Review Status
Reviewed
Scholarly Level
Faculty
Copyright Holder
Wiley
Rights URI
http://creativecommons.org/licenses/by-nc-nd/4.0/
Aggregated Source Repository
DSpace

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Attribution-NonCommercial-NoDerivatives 4.0 International