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Development of a Rapid Loop-Mediated Isothermal Amplification Assay for the Detection of Dothistroma septosporum Myrholm, Colin L.; Tomm, Bradley D.; Heinzelmann, Renate; Feau, Nicolas; Hamelin, Richard C.; McDougal, Rebecca; Winkworth, Richard C.; Ramsfield, Tod D.

Abstract

A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for the detection of the pine pathogen Dothistroma septosporum (G. Dorog.) M. Morelet. The specificity of the LAMP assay was tested using a selection of pine needle fungi, including Dothistroma pini Hulbary, and Lecanosticta acicola (Thüm.) Syd.; only D. septosporum DNA was amplified by the test. In terms of sensitivity, the assay was able to detect as little as 1 pg of total D. septosporum DNA. This assay enables DNA extracted from diseased host needles to be rapidly tested for the presence of D. septosporum using relatively simple to operate equipment away from a fully equipped molecular biology laboratory.

Item Metadata

Title
Development of a Rapid Loop-Mediated Isothermal Amplification Assay for the Detection of Dothistroma septosporum
Creator
Myrholm, Colin L.; Tomm, Bradley D.; Heinzelmann, Renate; Feau, Nicolas; Hamelin, Richard C.; McDougal, Rebecca; Winkworth, Richard C.; Ramsfield, Tod D.
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2021-03-19
Description
A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for the detection of the pine pathogen Dothistroma septosporum (G. Dorog.) M. Morelet. The specificity of the LAMP assay was tested using a selection of pine needle fungi, including Dothistroma pini Hulbary, and Lecanosticta acicola (Thüm.) Syd.; only D. septosporum DNA was amplified by the test. In terms of sensitivity, the assay was able to detect as little as 1 pg of total D. septosporum DNA. This assay enables DNA extracted from diseased host needles to be rapidly tested for the presence of D. septosporum using relatively simple to operate equipment away from a fully equipped molecular biology laboratory.
Subject
DNA-based diagnostics; LAMP; Dothistroma needle blight
Genre
Article
Type
Text
Language
eng
Date Available
2021-03-26
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 4.0
DOI
10.14288/1.0396399
URI
http://hdl.handle.net/2429/77645
Affiliation
Forestry, Faculty of; Non UBC; Forest and Conservation Sciences, Department of
Citation
Forests 12 (3): 362 (2021)
Publisher DOI
10.3390/f12030362
Peer Review Status
Reviewed
Scholarly Level
Faculty
Rights URI
https://creativecommons.org/licenses/by/4.0/
Aggregated Source Repository
DSpace

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CC BY 4.0