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Bio-Phototransistors with Immobilized Photosynthetic Proteins Takshi, Arash; Yaghoubi, Houman; Jun, Daniel; Beatty, J. Thomas, 1949-

Abstract

The efficient mechanism of light capture by photosynthetic proteins allows for energy transfer and conversion to electrochemical energy at very low light intensities. In this work, reaction center (RC) proteins, or a core complex consisting of the RC encircled by light harvesting (LH1) proteins (RC-LH1) from photosynthetic bacteria, were immobilized on an insulating layer of an ion-sensitive field-effect transistor (ISFET) to build bio-photodetectors. The orientation of the RC proteins was controlled via application of a hybrid linker made of 10-carboxydecylphosphonic acid and cytochrome c that anchored the RCs to their electron donor side. Bio-phototransistors consisting of either the core RC or the RC-LH1 core complex were tested under white and monochromic light. The difference between the dark and light currents at different wavelengths are well-matched with the absorption spectrum of the photosynthetic proteins. The results show potential for the use of photosynthetic proteins in photodetectors.

Item Metadata

Title
Bio-Phototransistors with Immobilized Photosynthetic Proteins
Creator
Takshi, Arash; Yaghoubi, Houman; Jun, Daniel; Beatty, J. Thomas, 1949-
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2020-10-18
Description
The efficient mechanism of light capture by photosynthetic proteins allows for energy transfer and conversion to electrochemical energy at very low light intensities. In this work, reaction center (RC) proteins, or a core complex consisting of the RC encircled by light harvesting (LH1) proteins (RC-LH1) from photosynthetic bacteria, were immobilized on an insulating layer of an ion-sensitive field-effect transistor (ISFET) to build bio-photodetectors. The orientation of the RC proteins was controlled via application of a hybrid linker made of 10-carboxydecylphosphonic acid and cytochrome c that anchored the RCs to their electron donor side. Bio-phototransistors consisting of either the core RC or the RC-LH1 core complex were tested under white and monochromic light. The difference between the dark and light currents at different wavelengths are well-matched with the absorption spectrum of the photosynthetic proteins. The results show potential for the use of photosynthetic proteins in photodetectors.
Subject
bio-phototransistor; reaction center; rhodobacter sphaeroides
Genre
Article
Type
Text
Language
eng
Date Available
2020-11-03
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 4.0
DOI
10.14288/1.0394899
URI
http://hdl.handle.net/2429/76453
Affiliation
Science, Faculty of; Non UBC; Microbiology and Immunology, Department of
Citation
Electronics 9 (10): 1709 (2020)
Publisher DOI
10.3390/electronics9101709
Peer Review Status
Reviewed
Scholarly Level
Faculty; Researcher
Rights URI
https://creativecommons.org/licenses/by/4.0/
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CC BY 4.0