UBC Faculty Research and Publications

Inflexibility of the plasma miRNA response following a high-carbohydrate meal in overweight insulin-resistant women Ramzan, F.; D’Souza, R. F; Durainayagam, B. R; Milan, A. M; Roy, N. C; Kruger, M. C; Henry, C. J; Mitchell, C. J; Cameron-Smith, D.

Abstract

Context: Metabolic inflexibility is a characteristic of insulin resistance, limiting the ability to transiently regulate oxidative metabolism and gene expression in response to nutrient availability. Little is known of the flexibility of post-transcriptional regulation, including circulatory miRNAs (c-miRNAs). Design: The abundances of targeted c-miRNAs, with reported functions in metabolic regulation, were analysed in response to a high-carbohydrate meal in healthy weight insulin-sensitive (IS) and overweight insulin-resistant (IR) women. Participants: Age-matched healthy weight IS (n = 20, BMI = 24.3 ± 0.70) and overweight IR (n = 20, BMI = 28.6 ± 0.67) women. Methods: An abundance of c-miRNAs was quantified prior to and following a high-carbohydrate breakfast meal (2500 kJ; 50% carbohydrate, 20% fat and 27% protein). Target genes of the differentially regulated c-miRNA were measured in RNA extracted from circulatory peripheral blood mononuclear cells (PBMCs). Results: In healthy weight IS women, both miR-15a-5p (p = 0.03) and miR-17-5p (p 

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