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Comparable type I interferon score determination from PAXgene and Tempus whole blood RNA collection and isolation systems Lamot, Lovro; Niemietz, Iwona; Brown, Kelly L

Abstract

Objective: Type I interferons (IFN) have important roles in many immune-mediated inflammatory diseases (IMIDs) and are a relatively new therapeutic target. Direct detection of type I IFNs has proved challenging, thus their presence is often inferred from the expression of interferon-stimulated genes (ISGs) and calculation of an interferon score (IS). The objective of this research was to determine if the expression of six common ISGs and subsequent IS were comparable when RNA was derived from the Tempus and PAXgene whole blood RNA collection systems. Results: Whole blood was obtained from ten healthy adults, incubated ex vivo in the absence and presence of recombinant human IFNα then divided into PAXgene and Tempus tubes. Despite reports of tube-specific patterns of gene expression, quantitative PCR (qPCR) analysis revealed no significant differences between PAXgene and Tempus tubes in either the homeostatic or IFNα-induced expression of six ISGs (IFI27, IFI44L, IFIT1, ISG15, RSAD2, SIGLEC1). Overall there was a strong correlation in the IS between unstimulated (r = 0.92, p = 0.0005) and IFNα-stimulated (r = 0.71, p = 0.0268) samples derived from the PAXgene and Tempus tubes.

Item Metadata

Title
Comparable type I interferon score determination from PAXgene and Tempus whole blood RNA collection and isolation systems
Creator
Lamot, Lovro; Niemietz, Iwona; Brown, Kelly L
Contributor
Children's Hospital (Vancouver, B.C.). Research Institute; University of British Columbia. Centre for Blood Research
Publisher
BioMed Central
Date Issued
2019-08-15
Description
Objective: Type I interferons (IFN) have important roles in many immune-mediated inflammatory diseases (IMIDs) and are a relatively new therapeutic target. Direct detection of type I IFNs has proved challenging, thus their presence is often inferred from the expression of interferon-stimulated genes (ISGs) and calculation of an interferon score (IS). The objective of this research was to determine if the expression of six common ISGs and subsequent IS were comparable when RNA was derived from the Tempus and PAXgene whole blood RNA collection systems. Results: Whole blood was obtained from ten healthy adults, incubated ex vivo in the absence and presence of recombinant human IFNα then divided into PAXgene and Tempus tubes. Despite reports of tube-specific patterns of gene expression, quantitative PCR (qPCR) analysis revealed no significant differences between PAXgene and Tempus tubes in either the homeostatic or IFNα-induced expression of six ISGs (IFI27, IFI44L, IFIT1, ISG15, RSAD2, SIGLEC1). Overall there was a strong correlation in the IS between unstimulated (r = 0.92, p = 0.0005) and IFNα-stimulated (r = 0.71, p = 0.0268) samples derived from the PAXgene and Tempus tubes.
Subject
Interferon score; Interferon stimulated genes; PAXgene; Tempus; RNA
Genre
Article
Type
Text
Language
eng
Date Available
2019-08-15
Provider
Vancouver : University of British Columbia Library
Rights
Attribution 4.0 International (CC BY 4.0)
DOI
10.14288/1.0380454
URI
http://hdl.handle.net/2429/71299
Affiliation
Medicine, Faculty of; Science, Faculty of; Other UBC; Medicine, Department of; Microbiology and Immunology, Department of; Pediatrics, Department of; Rheumatology, Division of
Citation
BMC Research Notes. 2019 Aug 15;12(1):511
Publisher DOI
10.1186/s13104-019-4562-z
Peer Review Status
Reviewed
Scholarly Level
Faculty
Copyright Holder
The Author(s)
Rights URI
http://creativecommons.org/licenses/by/4.0/
Aggregated Source Repository
DSpace

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Attribution 4.0 International (CC BY 4.0)