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The Genome of the Steller Sea Lion (Eumetopias jubatus) Kwan, Harwood H.; Culibrk, Luka; Taylor, Gregory A.; Leelakumari, Sreeja; Tan, Ryan; Jackman, Shaun D.; Tse, Kane; MacLeod, Tina; Cheng, Dean; Chuah, Eric; Kirk, Heather; Pandoh, Pawan; Carlsen, Rebecca; Zhao, Yongjun; Mungall, Andrew J.; Moore, Richard; Birol, Inanc; Marra, Marco, 1966-; Rosen, David A. S.; Haulena, Martin; Jones, Steven J. M.

Abstract

The Steller sea lion is the largest member of the Otariidae family and is found in the coastal waters of the northern Pacific Rim. Here, we present the Steller sea lion genome, determined through DNA sequencing approaches that utilized microfluidic partitioning library construction, as well as nanopore technologies. These methods constructed a highly contiguous assembly with a scaffold N50 length of over 14 megabases, a contig N50 length of over 242 kilobases and a total length of 2.404 gigabases. As a measure of completeness, 95.1% of 4104 highly conserved mammalian genes were found to be complete within the assembly. Further annotation identified 19,668 protein coding genes. The assembled genome sequence and underlying sequence data can be found at the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJNA475770.

Item Metadata

Title
The Genome of the Steller Sea Lion (Eumetopias jubatus)
Creator
Kwan, Harwood H.; Culibrk, Luka; Taylor, Gregory A.; Leelakumari, Sreeja; Tan, Ryan; Jackman, Shaun D.; Tse, Kane; MacLeod, Tina; Cheng, Dean; Chuah, Eric; Kirk, Heather; Pandoh, Pawan; Carlsen, Rebecca; Zhao, Yongjun; Mungall, Andrew J.; Moore, Richard; Birol, Inanc; Marra, Marco, 1966-; Rosen, David A. S.; Haulena, Martin; Jones, Steven J. M.
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2019-06-26
Description
The Steller sea lion is the largest member of the Otariidae family and is found in the coastal waters of the northern Pacific Rim. Here, we present the Steller sea lion genome, determined through DNA sequencing approaches that utilized microfluidic partitioning library construction, as well as nanopore technologies. These methods constructed a highly contiguous assembly with a scaffold N50 length of over 14 megabases, a contig N50 length of over 242 kilobases and a total length of 2.404 gigabases. As a measure of completeness, 95.1% of 4104 highly conserved mammalian genes were found to be complete within the assembly. Further annotation identified 19,668 protein coding genes. The assembled genome sequence and underlying sequence data can be found at the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJNA475770.
Subject
Steller sea lion; Eumetopias jubatus; genome; microfluidic partitioning; nanopore; marine animal
Genre
Article
Type
Text
Language
eng
Date Available
2019-07-26
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 4.0
DOI
10.14288/1.0380184
URI
http://hdl.handle.net/2429/71106
Affiliation
Medicine, Faculty of; Science, Faculty of; Non UBC; Medical Genetics, Department of; Oceans and Fisheries, Institute for the
Citation
Genes 10 (7): 486 (2019)
Publisher DOI
10.3390/genes10070486
Peer Review Status
Reviewed
Scholarly Level
Faculty
Rights URI
https://creativecommons.org/licenses/by/4.0/
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DSpace

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CC BY 4.0