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The Sequence of Two Bacteriophages with Hypermodified Bases Reveals Novel Phage-Host Interactions Kropinski, Andrew Maitland Boleslaw; Turner, Dann; Nash, John H. E.; Ackermann, Hans-Wolfgang; Lingohr, Erika J.; Warren, Richard A.; Ehrlich, Kenneth C.; Ehrlich, Melanie

Abstract

Bacteriophages SP-15 and ΦW-14 are members of the Myoviridae infecting Bacillus subtilis and Delftia (formerly Pseudomonas) acidovorans, respectively. What links them is that in both cases, approximately 50% of the thymine residues are replaced by hypermodified bases. The consequence of this is that the physico-chemical properties of the DNA are radically altered (melting temperature (Tm), buoyant density and susceptibility to restriction endonucleases). Using 454 pyrosequencing technology, we sequenced the genomes of both viruses. Phage ΦW-14 possesses a 157-kb genome (56.3% GC) specifying 236 proteins, while SP-15 is larger at 222 kb (38.6 mol % G + C) and encodes 318 proteins. In both cases, the phages can be considered genomic singletons since they do not possess BLASTn homologs. While no obvious genes were identified as being responsible for the modified base in ΦW-14, SP-15 contains a cluster of genes obviously involved in carbohydrate metabolism.

Item Metadata

Title
The Sequence of Two Bacteriophages with Hypermodified Bases Reveals Novel Phage-Host Interactions
Creator
Kropinski, Andrew Maitland Boleslaw; Turner, Dann; Nash, John H. E.; Ackermann, Hans-Wolfgang; Lingohr, Erika J.; Warren, Richard A.; Ehrlich, Kenneth C.; Ehrlich, Melanie
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2018-04-24
Description
Bacteriophages SP-15 and ΦW-14 are members of the Myoviridae infecting Bacillus subtilis and Delftia (formerly Pseudomonas) acidovorans, respectively. What links them is that in both cases, approximately 50% of the thymine residues are replaced by hypermodified bases. The consequence of this is that the physico-chemical properties of the DNA are radically altered (melting temperature (Tm), buoyant density and susceptibility to restriction endonucleases). Using 454 pyrosequencing technology, we sequenced the genomes of both viruses. Phage ΦW-14 possesses a 157-kb genome (56.3% GC) specifying 236 proteins, while SP-15 is larger at 222 kb (38.6 mol % G + C) and encodes 318 proteins. In both cases, the phages can be considered genomic singletons since they do not possess BLASTn homologs. While no obvious genes were identified as being responsible for the modified base in ΦW-14, SP-15 contains a cluster of genes obviously involved in carbohydrate metabolism.
Subject
Bacteriophage; Hypermodified bases; Alpha-putrescinylthymine; ΦW-14; SP-15; Bacillus; Delftia; DNA sequencing; 5-hydroxymethyluracil; 5-hydroxypentyluracil
Genre
Article
Type
Text
Language
eng
Date Available
2019-06-25
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 4.0
DOI
10.14288/1.0379589
URI
http://hdl.handle.net/2429/70793
Affiliation
Science, Faculty of; Non UBC; Microbiology and Immunology, Department of
Citation
Viruses 10 (5): 217 (2018)
Publisher DOI
10.3390/v10050217
Peer Review Status
Reviewed
Scholarly Level
Faculty
Rights URI
https://creativecommons.org/licenses/by/4.0/
Aggregated Source Repository
DSpace

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CC BY 4.0