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Abaxial Greening Phenotype in Hybrid Aspen Nowak, Julia S.; Douglas, Carl J.; Cronk, Quentin C. B.

Abstract

The typical angiosperm leaf, as in Arabidopsis, is bifacial consisting of top (adaxial) and bottom (abaxial) surfaces readily distinguishable by the underlying cell type (palisade and spongy mesophyll, respectively). Species of the genus Populus have leaves that are either conventionally bifacial or isobilateral. Isobilateral leaves have palisade mesophyll on the top and bottom of the leaf, making the two sides virtually indistinguishable at the macroscopic level. In poplars this has been termed the “abaxial greening” phenotype. Previous work has implicated ASYMMETRIC LEAVES1 (AS1) as an essential determinant of palisade mesophyll development. This gene, as well as other genes (84 in all) putatively involved in setting the dorsiventral axis of leaves, were investigated in two Populus species: black cottonwood (Populus trichocarpa) and hybrid aspen (P. tremula x tremuloides), representative of each leaf type (bifacial and isobilateral, respectively). Poplar orthologs of AS1 have significantly higher expression in aspen leaf blade and lower in the petiole, suggestive of a potential role in the isobilateral leaf phenotype consistent with the previously observed phenotypes. Furthermore, an ABERRANT TESTA SHAPE (ATS) ortholog has significantly lower expression in aspen leaf tissue, also suggesting a possible contribution of this gene to abaxial greening.

Item Metadata

Title
Abaxial Greening Phenotype in Hybrid Aspen
Creator
Nowak, Julia S.; Douglas, Carl J.; Cronk, Quentin C. B.
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2013-04-24
Description
The typical angiosperm leaf, as in Arabidopsis, is bifacial consisting of top (adaxial) and bottom (abaxial) surfaces readily distinguishable by the underlying cell type (palisade and spongy mesophyll, respectively). Species of the genus Populus have leaves that are either conventionally bifacial or isobilateral. Isobilateral leaves have palisade mesophyll on the top and bottom of the leaf, making the two sides virtually indistinguishable at the macroscopic level. In poplars this has been termed the “abaxial greening” phenotype. Previous work has implicated ASYMMETRIC LEAVES1 (AS1) as an essential determinant of palisade mesophyll development. This gene, as well as other genes (84 in all) putatively involved in setting the dorsiventral axis of leaves, were investigated in two Populus species: black cottonwood (Populus trichocarpa) and hybrid aspen (P. tremula x tremuloides), representative of each leaf type (bifacial and isobilateral, respectively). Poplar orthologs of AS1 have significantly higher expression in aspen leaf blade and lower in the petiole, suggestive of a potential role in the isobilateral leaf phenotype consistent with the previously observed phenotypes. Furthermore, an ABERRANT TESTA SHAPE (ATS) ortholog has significantly lower expression in aspen leaf tissue, also suggesting a possible contribution of this gene to abaxial greening.
Subject
Adaxial–abaxial polarity; Populus trichocarpa; Populus tremula x tremuloides; ASYMMETRIC LEAVES1; Abaxial greenness; KANADI; Leaf blade
Genre
Article
Type
Text
Language
eng
Date Available
2019-04-15
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 3.0
DOI
10.14288/1.0378221
URI
http://hdl.handle.net/2429/69701
Affiliation
Science, Faculty of; Non UBC; Botany, Department of
Citation
Plants 2 (2): 279-301 (2013)
Publisher DOI
10.3390/plants2020279
Peer Review Status
Reviewed
Scholarly Level
Faculty
Rights URI
https://creativecommons.org/licenses/by-nc-sa/3.0/
Aggregated Source Repository
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CC BY 3.0