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Genetic Analysis of Health-Related Secondary Metabolites in a Brassica rapa Recombinant Inbred Line Population Bagheri, Hedayat; El-Soda, Mohamed; Kim, Hye Kyong; Fritsche, Steffi; Jung, Christian; Aarts, Mark G. M.

Abstract

The genetic basis of the wide variation for nutritional traits in Brassica rapa is largely unknown. A new Recombinant Inbred Line (RIL) population was profiled using High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR) analysis to detect quantitative trait loci (QTLs) controlling seed tocopherol and seedling metabolite concentrations. RIL population parent L58 had a higher level of glucosinolates and phenylpropanoids, whereas levels of sucrose, glucose and glutamate were higher in the other RIL population parent, R-o-18. QTL related to seed tocopherol (α-, β-, γ-, δ-, α-⁄γ- and total tocopherol) concentrations were detected on chromosomes A3, A6, A9 and A10, explaining 11%–35% of the respective variation. The locus on A3 co-locates with the BrVTE1gene, encoding tocopherol cyclase. NMR spectroscopy identified the presence of organic/amino acid, sugar/glucosinolate and aromatic compounds in seedlings. QTL positions were obtained for most of the identified compounds. Compared to previous studies, novel loci were found for glucosinolate concentrations. This work can be used to design markers for marker-assisted selection of nutritional compounds in B. rapa.

Item Metadata

Title
Genetic Analysis of Health-Related Secondary Metabolites in a Brassica rapa Recombinant Inbred Line Population
Creator
Bagheri, Hedayat; El-Soda, Mohamed; Kim, Hye Kyong; Fritsche, Steffi; Jung, Christian; Aarts, Mark G. M.
Publisher
Multidisciplinary Digital Publishing Institute
Date Issued
2013-07-25
Description
The genetic basis of the wide variation for nutritional traits in Brassica rapa is largely unknown. A new Recombinant Inbred Line (RIL) population was profiled using High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR) analysis to detect quantitative trait loci (QTLs) controlling seed tocopherol and seedling metabolite concentrations. RIL population parent L58 had a higher level of glucosinolates and phenylpropanoids, whereas levels of sucrose, glucose and glutamate were higher in the other RIL population parent, R-o-18. QTL related to seed tocopherol (α-, β-, γ-, δ-, α-⁄γ- and total tocopherol) concentrations were detected on chromosomes A3, A6, A9 and A10, explaining 11%–35% of the respective variation. The locus on A3 co-locates with the BrVTE1gene, encoding tocopherol cyclase. NMR spectroscopy identified the presence of organic/amino acid, sugar/glucosinolate and aromatic compounds in seedlings. QTL positions were obtained for most of the identified compounds. Compared to previous studies, novel loci were found for glucosinolate concentrations. This work can be used to design markers for marker-assisted selection of nutritional compounds in B. rapa.
Subject
Brassica rapa; Secondary metabolites; Nutritional value; Quantitative Trait Loci; Tocopherol; Glucosinolates
Genre
Article
Type
Text
Language
eng
Date Available
2019-04-08
Provider
Vancouver : University of British Columbia Library
Rights
CC BY 3.0
DOI
10.14288/1.0378031
URI
http://hdl.handle.net/2429/69529
Affiliation
Science, Faculty of; Botany, Department of
Citation
International Journal of Molecular Sciences 14 (8): 15561-15577 (2013)
Publisher DOI
10.3390/ijms140815561
Peer Review Status
Reviewed
Scholarly Level
Faculty
Rights URI
https://creativecommons.org/licenses/by-nc-sa/3.0/
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CC BY 3.0