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Genome-wide mapping of imprinted differentially methylated regions by DNA methylation profiling of human placentas from triploidies Yuen, Ryan K; Jiang, Ruby; Peñaherrera, Maria S; McFadden, Deborah E; Robinson, Wendy P

Abstract

Background: Genomic imprinting is an important epigenetic process involved in regulating placental and foetal growth. Imprinted genes are typically associated with differentially methylated regions (DMRs) whereby one of the two alleles is DNA methylated depending on the parent of origin. Identifying imprinted DMRs in humans is complicated by species- and tissue-specific differences in imprinting status and the presence of multiple regulatory regions associated with a particular gene, only some of which may be imprinted. In this study, we have taken advantage of the unbalanced parental genomic constitutions in triploidies to further characterize human DMRs associated with known imprinted genes and identify novel imprinted DMRs. Results: By comparing the promoter methylation status of over 14,000 genes in human placentas from ten diandries (extra paternal haploid set) and ten digynies (extra maternal haploid set) and using 6 complete hydatidiform moles (paternal origin) and ten chromosomally normal placentas for comparison, we identified 62 genes with apparently imprinted DMRs (false discovery rate

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Title
Genome-wide mapping of imprinted differentially methylated regions by DNA methylation profiling of human placentas from triploidies
Creator
Yuen, Ryan K; Jiang, Ruby; Peñaherrera, Maria S; McFadden, Deborah E; Robinson, Wendy P
Contributor
Child and Family Research Institute
Publisher
BioMed Central
Date Issued
2011-07-13
Description
Background: Genomic imprinting is an important epigenetic process involved in regulating placental and foetal growth. Imprinted genes are typically associated with differentially methylated regions (DMRs) whereby one of the two alleles is DNA methylated depending on the parent of origin. Identifying imprinted DMRs in humans is complicated by species- and tissue-specific differences in imprinting status and the presence of multiple regulatory regions associated with a particular gene, only some of which may be imprinted. In this study, we have taken advantage of the unbalanced parental genomic constitutions in triploidies to further characterize human DMRs associated with known imprinted genes and identify novel imprinted DMRs. Results: By comparing the promoter methylation status of over 14,000 genes in human placentas from ten diandries (extra paternal haploid set) and ten digynies (extra maternal haploid set) and using 6 complete hydatidiform moles (paternal origin) and ten chromosomally normal placentas for comparison, we identified 62 genes with apparently imprinted DMRs (false discovery rate
Genre
Article
Type
Text
Language
eng
Date Available
2016-02-10
Provider
Vancouver : University of British Columbia Library
Rights
Attribution 4.0 International (CC BY 4.0)
DOI
10.14288/1.0224070
URI
http://hdl.handle.net/2429/56955
Affiliation
Medical Genetics, Department of; Medicine, Faculty of; Pathology and Laboratory Medicine, Department of; Other UBC; Non UBC
Citation
Epigenetics & Chromatin. 2011 Jul 13;4(1):10
Publisher DOI
10.1186/1756-8935-4-10
Peer Review Status
Reviewed
Scholarly Level
Faculty
Copyright Holder
Yuen et al; licensee BioMed Central Ltd.
Rights URI
http://creativecommons.org/licenses/by/4.0/
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Attribution 4.0 International (CC BY 4.0)