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Genome-wide mapping of imprinted differentially methylated regions by DNA methylation profiling of human placentas from triploidies Yuen, Ryan K; Jiang, Ruby; Peñaherrera, Maria S; McFadden, Deborah E; Robinson, Wendy P
Abstract
Background: Genomic imprinting is an important epigenetic process involved in regulating placental and foetal growth. Imprinted genes are typically associated with differentially methylated regions (DMRs) whereby one of the two alleles is DNA methylated depending on the parent of origin. Identifying imprinted DMRs in humans is complicated by species- and tissue-specific differences in imprinting status and the presence of multiple regulatory regions associated with a particular gene, only some of which may be imprinted. In this study, we have taken advantage of the unbalanced parental genomic constitutions in triploidies to further characterize human DMRs associated with known imprinted genes and identify novel imprinted DMRs. Results: By comparing the promoter methylation status of over 14,000 genes in human placentas from ten diandries (extra paternal haploid set) and ten digynies (extra maternal haploid set) and using 6 complete hydatidiform moles (paternal origin) and ten chromosomally normal placentas for comparison, we identified 62 genes with apparently imprinted DMRs (false discovery rate
Item Metadata
Title |
Genome-wide mapping of imprinted differentially methylated regions by DNA methylation profiling of human placentas from triploidies
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Creator | |
Contributor | |
Publisher |
BioMed Central
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Date Issued |
2011-07-13
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Description |
Background:
Genomic imprinting is an important epigenetic process involved in regulating placental and foetal growth. Imprinted genes are typically associated with differentially methylated regions (DMRs) whereby one of the two alleles is DNA methylated depending on the parent of origin. Identifying imprinted DMRs in humans is complicated by species- and tissue-specific differences in imprinting status and the presence of multiple regulatory regions associated with a particular gene, only some of which may be imprinted. In this study, we have taken advantage of the unbalanced parental genomic constitutions in triploidies to further characterize human DMRs associated with known imprinted genes and identify novel imprinted DMRs.
Results:
By comparing the promoter methylation status of over 14,000 genes in human placentas from ten diandries (extra paternal haploid set) and ten digynies (extra maternal haploid set) and using 6 complete hydatidiform moles (paternal origin) and ten chromosomally normal placentas for comparison, we identified 62 genes with apparently imprinted DMRs (false discovery rate
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Type | |
Language |
eng
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Date Available |
2016-02-10
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Provider |
Vancouver : University of British Columbia Library
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Rights |
Attribution 4.0 International (CC BY 4.0)
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DOI |
10.14288/1.0224070
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URI | |
Affiliation | |
Citation |
Epigenetics & Chromatin. 2011 Jul 13;4(1):10
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Publisher DOI |
10.1186/1756-8935-4-10
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Peer Review Status |
Reviewed
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Scholarly Level |
Faculty
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Copyright Holder |
Yuen et al; licensee BioMed Central Ltd.
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Rights URI | |
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DSpace
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Item Citations and Data
Rights
Attribution 4.0 International (CC BY 4.0)