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Requirement of epithelial integrin-linked kinase for facilitation of Citrobacter rodentium-induced colitis Assi, Kiran; Bergstrom, Kirk; Vallance, Bruce; Owen, David; Salh, Baljinder

Abstract

Background: Integrin-linked kinase (ILK) is a serine-threonine kinase that transduces extracellular matrix-related cues into intracellular signals, with fundamental roles in cell motility, development and cancer. Recently ILK been shown to have an important role in bacterial epithelial cell attachment, through ILK-bacterial OspE binding. Here we report on the role of epithelial derived ILK in response to Citrobacter rodentium infection. Methods: C. rodentium was administered to both control and intestinal epithelial cell ILK knockout mice. Histological inflammatory scores were assessed, and cytokines measured by ELISA as well as RT-PCR, in mouse colons. Bacterial colonization was determined by plating homogenates onto MacConkey agar, and immunofluorescence microscopy performed using anti-LPS and anti-Tir antibodies. Results: ILK-ko mice exhibited reduced weight loss at 15 days post-infection (p 

Item Metadata

Title
Requirement of epithelial integrin-linked kinase for facilitation of Citrobacter rodentium-induced colitis
Creator
Assi, Kiran; Bergstrom, Kirk; Vallance, Bruce; Owen, David; Salh, Baljinder
Contributor
Child and Family Research Institute
Publisher
BioMed Central
Date Issued
2013-09-11
Description
Background: Integrin-linked kinase (ILK) is a serine-threonine kinase that transduces extracellular matrix-related cues into intracellular signals, with fundamental roles in cell motility, development and cancer. Recently ILK been shown to have an important role in bacterial epithelial cell attachment, through ILK-bacterial OspE binding. Here we report on the role of epithelial derived ILK in response to Citrobacter rodentium infection. Methods: C. rodentium was administered to both control and intestinal epithelial cell ILK knockout mice. Histological inflammatory scores were assessed, and cytokines measured by ELISA as well as RT-PCR, in mouse colons. Bacterial colonization was determined by plating homogenates onto MacConkey agar, and immunofluorescence microscopy performed using anti-LPS and anti-Tir antibodies. Results: ILK-ko mice exhibited reduced weight loss at 15 days post-infection (p 
Subject
ILK; Citrobacter; Colitis; Fibronectin
Genre
Article
Type
Text
Language
eng
Date Available
2016-01-18
Provider
Vancouver : University of British Columbia Library
Rights
Attribution 4.0 International (CC BY 4.0)
DOI
10.14288/1.0223749
URI
http://hdl.handle.net/2429/56577
Affiliation
Gastroenterology, Division of; Medicine, Department of; Medicine, Faculty of; Other UBC; Non UBC
Citation
BMC Gastroenterology. 2013 Sep 11;13(1):137
Publisher DOI
10.1186/1471-230X-13-137
Peer Review Status
Reviewed
Scholarly Level
Faculty
Copyright Holder
Kiran et al.; licensee BioMed Central Ltd.
Rights URI
http://creativecommons.org/licenses/by/4.0/
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Attribution 4.0 International (CC BY 4.0)