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Requirement of epithelial integrin-linked kinase for facilitation of Citrobacter rodentium-induced colitis Assi, Kiran; Bergstrom, Kirk; Vallance, Bruce; Owen, David; Salh, Baljinder
Abstract
Background: Integrin-linked kinase (ILK) is a serine-threonine kinase that transduces extracellular matrix-related cues into intracellular signals, with fundamental roles in cell motility, development and cancer. Recently ILK been shown to have an important role in bacterial epithelial cell attachment, through ILK-bacterial OspE binding. Here we report on the role of epithelial derived ILK in response to Citrobacter rodentium infection. Methods: C. rodentium was administered to both control and intestinal epithelial cell ILK knockout mice. Histological inflammatory scores were assessed, and cytokines measured by ELISA as well as RT-PCR, in mouse colons. Bacterial colonization was determined by plating homogenates onto MacConkey agar, and immunofluorescence microscopy performed using anti-LPS and anti-Tir antibodies. Results: ILK-ko mice exhibited reduced weight loss at 15 days post-infection (p
Item Metadata
Title |
Requirement of epithelial integrin-linked kinase for facilitation of Citrobacter rodentium-induced colitis
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Creator | |
Contributor | |
Publisher |
BioMed Central
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Date Issued |
2013-09-11
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Description |
Background:
Integrin-linked kinase (ILK) is a serine-threonine kinase that transduces extracellular matrix-related cues into intracellular signals, with fundamental roles in cell motility, development and cancer. Recently ILK been shown to have an important role in bacterial epithelial cell attachment, through ILK-bacterial OspE binding. Here we report on the role of epithelial derived ILK in response to Citrobacter rodentium infection.
Methods:
C. rodentium was administered to both control and intestinal epithelial cell ILK knockout mice. Histological inflammatory scores were assessed, and cytokines measured by ELISA as well as RT-PCR, in mouse colons. Bacterial colonization was determined by plating homogenates onto MacConkey agar, and immunofluorescence microscopy performed using anti-LPS and anti-Tir antibodies.
Results:
ILK-ko mice exhibited reduced weight loss at 15 days post-infection (p
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Subject | |
Genre | |
Type | |
Language |
eng
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Date Available |
2016-01-18
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Provider |
Vancouver : University of British Columbia Library
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Rights |
Attribution 4.0 International (CC BY 4.0)
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DOI |
10.14288/1.0223749
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URI | |
Affiliation | |
Citation |
BMC Gastroenterology. 2013 Sep 11;13(1):137
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Publisher DOI |
10.1186/1471-230X-13-137
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Peer Review Status |
Reviewed
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Scholarly Level |
Faculty
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Copyright Holder |
Kiran et al.; licensee BioMed Central Ltd.
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Rights URI | |
Aggregated Source Repository |
DSpace
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Item Citations and Data
Rights
Attribution 4.0 International (CC BY 4.0)