UBC Faculty Research and Publications

Requirement of epithelial integrin-linked kinase for facilitation of Citrobacter rodentium-induced colitis Assi, Kiran; Bergstrom, Kirk; Vallance, Bruce; Owen, David; Salh, Baljinder


Background: Integrin-linked kinase (ILK) is a serine-threonine kinase that transduces extracellular matrix-related cues into intracellular signals, with fundamental roles in cell motility, development and cancer. Recently ILK been shown to have an important role in bacterial epithelial cell attachment, through ILK-bacterial OspE binding. Here we report on the role of epithelial derived ILK in response to Citrobacter rodentium infection. Methods: C. rodentium was administered to both control and intestinal epithelial cell ILK knockout mice. Histological inflammatory scores were assessed, and cytokines measured by ELISA as well as RT-PCR, in mouse colons. Bacterial colonization was determined by plating homogenates onto MacConkey agar, and immunofluorescence microscopy performed using anti-LPS and anti-Tir antibodies. Results: ILK-ko mice exhibited reduced weight loss at 15 days post-infection (p 

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