- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Faculty Research and Publications /
- Discovery of novel alternatively spliced C. elegans...
Open Collections
UBC Faculty Research and Publications
Discovery of novel alternatively spliced C. elegans transcripts by computational analysis of SAGE data Ruzanov, Peter; Jones, Steven J. M.; Riddle, Donald L.
Abstract
Background: Alternative RNA splicing allows cells to produce multiple protein isoforms from one gene. These isoforms may have specialized functions, and may be tissue- or stage-specific. Our aim was to use computational analysis of SAGE and genomic data to predict alternatively spliced transcripts expressed in C. elegans. Results We predicted novel alternatively spliced variants and confirmed five of eighteen candidates selected for experimental validation by RT-PCR tests and DNA sequencing. Conclusion We show that SAGE data can be efficiently used to discover alternative mRNA isoforms, including those with skipped exons or retained introns. Our results also imply that C. elegans may produce a larger number of alternatively spliced transcripts than initially estimated.
Item Metadata
Title |
Discovery of novel alternatively spliced C. elegans transcripts by computational analysis of SAGE data
|
Creator | |
Contributor | |
Publisher |
BioMed Central
|
Date Issued |
2007-11-30
|
Description |
Background:
Alternative RNA splicing allows cells to produce multiple protein isoforms from one gene. These isoforms may have specialized functions, and may be tissue- or stage-specific. Our aim was to use computational analysis of SAGE and genomic data to predict alternatively spliced transcripts expressed in C. elegans.
Results
We predicted novel alternatively spliced variants and confirmed five of eighteen candidates selected for experimental validation by RT-PCR tests and DNA sequencing.
Conclusion
We show that SAGE data can be efficiently used to discover alternative mRNA isoforms, including those with skipped exons or retained introns. Our results also imply that C. elegans may produce a larger number of alternatively spliced transcripts than initially estimated.
|
Genre | |
Type | |
Language |
eng
|
Date Available |
2015-11-05
|
Provider |
Vancouver : University of British Columbia Library
|
Rights |
Attribution 4.0 International (CC BY 4.0)
|
DOI |
10.14288/1.0167788
|
URI | |
Affiliation | |
Citation |
BMC Genomics. 2007 Nov 30;8(1):447
|
Publisher DOI |
10.1186/1471-2164-8-447
|
Peer Review Status |
Reviewed
|
Scholarly Level |
Faculty
|
Copyright Holder |
Ruzanov et al.
|
Rights URI | |
Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
Attribution 4.0 International (CC BY 4.0)