Unraveling mannan biosynthesis in poplar and spruce : functional characterization of cellulose synthase-Like family A (CSLA) and guanosine-diphosphate-mannose (GMP) genes in poplar
department or school: Forestry, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Guevara Rozo, Sydne
issued: 2024-05-09T18:02:29Z
issued: 2024
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Lignocellulosic feedstocks are pivotal to the transition to a biobased economy, however, the plant cell wall evolved to withstand stresses, microbial attack, and degradation, and therefore presents significant challenges for industrial biomass processing. Structural modifications of xylan, the major hemicellulose in angiosperms, have been shown to positively affect cell wall deconstruction by interfering with xylan-cellulose-lignin interactions. In contrast, the role of mannan, a major hemicellulose commonly found in gymnosperms (minor hemicellulose in angiosperm cell walls), which also interacts with cellulose and lignin, is still poorly understood. Genes involved in the mannan biosynthetic pathway have been described in Arabidopsis, where cellulose synthase-like family A (CSLA) proteins synthesize the mannan backbone. The functional validation of orthologous genes responsible for the synthesis of this polysaccharide in woody plants and the effects of changing its content on secondary cell wall stability remain largely unstudied. In this study, I identify genes from woody plants that are responsible for mannan biosynthesis and evaluate the effect of modifying the expression of these genes on plant growth and cell wall structure. Using a combination of bioinformatic and phylogenetic tools, I identified putative genes from spruce (Picea engelmannii × glauca × sitchensis; PG29) and poplar (Populus tremula × Populus alba; 717) that encode CSLAs and GDP-mannose pyrophosphorylase (GMP) proteins. I functionally characterized these genes by evaluating if they were able to restore mannan content in the Arabidopsis csla 2,3,9 and vtc1-1 mutants, respectively. Then, I evaluated their function in wood by mutating the poplar CSLA and GMP candidate genes via CRISPR Cas9 or ectopically expressing the spruce gene candidates in poplar. In this work, I demonstrate that mannan content can be manipulated in tree stems either by altering the expression of CSLA or GMP encoding genes, wi…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/88264
note: UNRAVELING MANNAN BIOSYNTHESIS IN POPLAR AND SPRUCE: FUNCTIONAL CHARACTERIZATION OF CELLULOSE SYNTHASE-LIKE FAMILY A (CSLA) AND GUANOSINE-DIPHOSPHATE-MANNOSE (GMP) GENES IN POPLAR by Sydne Guevara Rozo B.Sc., Universidad Nacional de Colombia, 2015 M.Sc., The University of Alberta, 2018 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Forestry) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) May 2024 © Sydne Guevara Rozo, 2024 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: Unraveling mannan biosynthesis in Poplar and Spruce: functional characterization of Cellulose Synthase-Like family A (CSLA) and Guanosine-diphosphate-Mannose (GMP) genes in Poplar submitted by Sydne Guevara Rozo in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Forestry Examining Committee: Dr. Shawn D. Mansfield, Professor, Wood Science, UBC Supervisor Dr. Lacey Samuels, Professor, Botany, UBC Supervisory Committee Member Dr. George Haughn, Professor, Botany, UBC Supervisory Committee Member Dr. Harry Brumer, Professor, Michael Smith Laboratories, UBC Supervisory Committee Member Dr. Joerg Bohlmann, Professor, Michael Smith Laboratories, UBC University Examiner Dr. Andrew Riseman, Professor, Plant Science, UBC University Examiner iii Abstract Lignocellulosic feedstocks are pivotal to the transition to a biobased economy, however, the plant cell wall evolved to withstand stresses, microbial attack, and degradation, and therefore presents significant challenges for industrial biomass processing. Structural modifications of xylan, the major hemicellulose in angiosperms, have been shown to positively affect cell wall deconstruction by interfering with xylan-cellulose-lignin interactions. In contrast, the role of mannan, a major hemicellulose common…
has type: Thesis/Dissertation
date issued: 2024-11
is shown at: 10.14288/1.0442424
language: eng
degree discipline: Forestry
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Mansfield, Shawn D., 1969-
type: Text
identifier URI: http://hdl.handle.net/2429/88264