Evaluation of the grapevine red blotch virus epidemiological landscape of the Okanagan valley
department or school: Science, Irving K. Barber Faculty of (Okanagan)
department or school: Biology, Department of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Kahl, Dieter
issued: 2021-08-01T07:00:00Z
issued: 2021
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: The first data chapter of this research presents a novel approach for identifying potential insect vectors of circulative plant viruses, significantly reducing time-to-results and the resource costs associated with greenhouse transmission experiments. This artificial transmission protocol using a buffered sucrose solution as the virus recipient in place of a living plant, also eliminates plant-to-plant variation in susceptibility to infection, allowing assessment of virus-insect compatibility required for successful transmission of persistent viruses. To validate this approach, species of hemipteran insects, including leafhoppers, froghoppers, aphids, sharpshooters, and treehoppers, were subjected to the artificial feeding system to determine their ability to vector Grapevine red blotch virus (GRBV). Test insects were allowed to feed on a potted grapevine infected with GRBV for three days and then transferred to tubes containing the sucrose solution partitioned by a thinly stretched Parafilm membrane. After three days of feeding through the membrane, viruliferous test insects were stored for species identification and the sucrose solutions were tested by polymerase chain reaction for the presence of GRBV DNA. Of all the insects tested (n = 393), only nine treehoppers from two different species successfully transmitted GRBV to the sucrose solutions, indicating a high-likelihood of vector capability to plants, to be validated by greenhouse or field experiments. The second data chapter presents research that investigated GRBV titres from several grapevine tissues, including roots, buds, cortical scrapings, and leaves, at varying distances from the cordons, and at different times during the growing season and winter dormancy. Two common nucleic acid extraction techniques were compared, a low cost high-throughput nucleic acid extraction method (GES) and a DNA column purification method. Ideal combinations of sampling parameters were determined for summer and winter diagn…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/78480
note: Evaluation of the Grapevine red blotch virus Epidemiological Landscape of the Okanagan Valley by Dieter Kahl B.Sc., University of Victoria 2018 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE COLLEGE OF GRADUATE STUDIES (Biology) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) May 2021 © Dieter Kahl 2021 ii The following individuals certify that they have read, and recommend to the College of Graduate Studies for acceptance, a thesis/dissertation entitled: Evaluation of the Grapevine red blotch virus Epidemiological Landscape of the Okanagan Valley submitted by Dieter Kahl in partial fulfillment of the requirements of the degree of Master of Science. Dr. Miranda Hart, Department of Biology, Irving K. Barber Faculty of Sciences Supervisor Dr. D. Thomas Lowery, Department of Biology, Irving K. Barber Faculty of Sciences Supervisory Committee Member Dr. José Ramón Úrbez-Torres, Department of Biology, Irving K. Barber Faculty of Sciences Supervisory Committee Member Dr. Jörg Bohlmann, Department of Botany, Faculty of Science, UBC Vancouver University Examiner Click or tap here to enter text. External Examiner Additional Committee Members include: Dr. Robert Lalonde, Department of Biology, Irving K. Barber Faculty of Sciences Supervisory Committee Member Click or tap here to enter text. Supervisory Committee Member iii Abstract The first data chapter of this research presents a novel approach for identifying potential insect vectors of circulative plant viruses, significantly reducing time-to-results and the resource costs associated with greenhouse transmission experiments. This artificial transmission protocol using a buffered sucrose solution as the virus recipient in place of a living plant, also eliminates plant-to-plant variation in susceptibility to infection, allowing assessment of virus-insect compatibility required for successful transmission of persistent viruses. To validate this approach, species of hemi…
has type: Thesis/Dissertation
date issued: 2021-09
is shown at: 10.14288/1.0398192
language: eng
degree discipline: Biology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Hart, Miranda
contributor: Lowery, Thomas
type: Text
identifier URI: http://hdl.handle.net/2429/78480