Characterisation of a novel pathway for ribosomal RNA maturation in Sulfolobus acidocaldarius
department or school: Medicine, Faculty of
department or school: Medical Genetics, Department of
data provider: DSpace
degree campus: UBCV
creator: Durovic, Peter Vincent
issued: 2012-03-16T19:23:21Z
issued: 1993
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Since the initial proposition that the archaebacteria form a primary kingdom as distinct as that of the eubacteria or the eukaryotes, sequence data generated from the ribosomal RNA genes have flooded the databases and periodicals. Phylogenetic trees based on these sequences have been constructed to map the finest details of topology and branching order within the archaebacteria. Yet, despite the plethora of sequence data, relatively little was discovered regarding rRNAgene regulation, transcript processing and requirements for mature ribosome function. The aim of this study is to analyze possible novel regulatory mechanisms in the rRNA genes of the extremely thermoacidophilic archaebacterium Sulfolobus acidoccddccrius. The three ribosomal RNA genes were cloned and sequenced. The gene organization was confirmed to differ from that of the halophilic archaebacteria and the eubacteria: the 5S gene was not linked to the 16S and 23S operon, and the operon lacked recognizable tRNA sequences. Southern hybridization unveiled, and sequence data confirmed a long-standing confusion regarding species identity. The previously published Sulfolobus acidocaldarius 5S sequence was shown to have been attributed to the wrong species. Mapping experiments showed that both transcripts initiated downstream of a previously defined archaebacteria! promoter sequence. While sequence data showed the 5S transcript start site and end site to be coincidental with the mature 5S termini, the 16S-23S transcript was shown to contain a 143 nucleotide transcribed leader sequence, a 138 nucleotide intergenic sequence, and a trailer sequence of at least 105 nucleotides. Inverted repeat sequences within these transcribed non-coding regions allow for the formation of numerous stem-loops conforming to a semi-conserved archaebacterial structure. While no processing took place within the 5S transcript, extensive processing of the 16S-23S transcript was observed. Of the 12 processing sites mapped, only 6 could …
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/41498
note: CHARACTERISATION OF A NOVEL PATHWAY FOR RIBOSOMAL RNA MATURATION IN SULFOWBUS ACIDOCAWARIUS by PETER VINCENT DUROVIC B.Sc., The University of British Columbia, 1986A THESIS SUBMITTED IN PARfIAL FULFILLMENT OF THE REQUIREMENT FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE STUDIES GENETICS GRADUATE PROGRAM We accept this thesis as conforming to the required standard THE UN�TY OF BRITISH COLUMBIA March 1993 © Peter Vincent Durovic, 1993 In presenting this thesis in partial fulfilment of the requirements for an advanced degree at the University of British Columbia, I agree that the Library shall make it freely available for reference and study. I further agree that permission for extensive copying of this thesis for scholarly purposes may be granted by the head of my department or by his or her representatives. It is understood that copying or publication of this thesis for financial gain shall not be allowed without my written permission. (Signature) Department of Biochemistry (Genetics Program) The University of British Columbia Vancouver, Canada A p r i l 30, 1993 -6 (2/88) ii ABSTRACT Since the initial proposition that the archaebacteria form a primary kingdom as distinct as that of the eubacteria or the eukaryotes, sequence data generated from the ribosomal RNA genes have flooded the databases and periodicals. Phylogenetic trees based on these sequences have been constructed to map the finest details of topology and branching order within the archaebacteria. Yet, despite the plethora of sequence data, relatively little was discovered regarding rRNAgene regulation, transcript processing and requirements for mature ribosome function. The aim of this study is to analyze possible novel regulatory mechanisms in the rRNA genes of the extremely thermoacidophilic archaebacterium Sulfolobus acidoccddccrius. The three ribosomal RNA genes were cloned and sequenced. The gene organization was confirmed to differ from that of the halophilic archaebacteria and …
has type: Thesis/Dissertation
is shown at: 10.14288/1.0106952
language: eng
degree discipline: Genetics
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/41498