The effect of 3-amino-1,2,4-triazole on the uptake, retention, distribution, and utilization of labelled phosphorus by young bean plants
department or school: Science, Faculty of
department or school: Botany, Department of
data provider: DSpace
degree campus: UBCV
creator: LaBerge, Donald Emmanuel
issued: 2012-01-13T20:19:08Z
issued: 1961
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Bean plants were grown in a phosphate-free nutrient solution to the early trifoliate stage. At this time, they were transferred to a minus phosphate nutrient solution containing 100 p.p.m. 3-amino-1,2,4-triazole for 48 hours, and then placed into a labelled phosphate nutrient solution for another hour. The plants were then returned to a phosphate-free nutrient solution and harvested one, 24, 48, and 96 hours after the period of initial phosphate uptake. AT-treatment did not affect uptake of P³² but did decrease loss of P³² to the phosphate-free nutrient solutions after it had been absorbed by the plants. The proportion of absorbed phosphate found in the stems and leaves of AT-treated plants was higher than in these organs in the control plants. This phosphate represented an increase in both acid-soluble activity and acid-insoluble activity. The accumulation of acid-soluble activity in the shoots of AT-treated plants was an accumulation of inorganic phosphates, sugar phosphates, and nucleotides. AT appeared to inhibit downward translocation of acid-soluble and acid-insoluble activity. The incorporation of P³² into esterified compounds (i.e., nucleotides and sugar phosphates) was unaffected by AT indicating that AT does not interfere with oxidative phosphorylation nor with glycolysis. However, AT did inhibit transfer of P³² from the acid-soluble fraction to the acid-insoluble fraction. Therefore, the principal effect of AT is to inhibit the incorporation of phosphate into one or more of the nucleic acid, phospholipid, or phosphoprotein fractions.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/40079
note: THE EFFECT OF 3-AMIN0-l ,2,4-TRIAZ0LE ON THE UPTAKE, RETENTION, DISTRIBUTION, AND UTILIZATION OF LABELLED PHOSPHORUS BY YOUNG- BEAN PLANTS. by Donald E . LaBerge B . S c . , . Univers i ty of B r i t i s h Columbia, 1959 A Thesis Submitted i n P a r t i a l Ful f i lment of the Requirements f o r the Degree of Master of Science In the Department of Botany We accept th i s thesis as conforming to the required standard The Univers i ty of B r i t i s h Columbia June,. 1961 In presenting t h i s t h e s i s i n p a r t i a l f u l f i l m e n t of the requirements f o r an advanced degree at the U n i v e r s i t y of B r i t i s h Columbia, I agree that the L i b r a r y s h a l l make i t f r e e l y a v a i l a b l e f o r reference and study. I f u r t h e r agree that permission f o r extensive copying of t h i s t h e s i s f o r s c h o l a r l y purposes may be granted by the Head of my Department or by h i s representatives. It i s understood that copying or p u b l i c a t i o n of t h i s t h e s i s f o r f i n a n c i a l gain s h a l l not be allowed without my written permission. Department Date *&k\ The U n i v e r s i t y of B r i t i s h Columbia, Vancouver 8 , Canada. ABSTRACT Bean plants were grown in a phosphate-free nutrient solution to the early t r i f o l i a t e stage. At this time, they were transferred to a minus phosphate nutrient solution containing 100 p.p.m. 3~amino-l,2,4-triazole for 48 hours, and then placed into a labelled phosphate nutrient solution for another hour. The plants were then returned to a phosphate-free nutrient solution and harvested one, 24, 48, and 96 hours after the period of i n i t i a l phosphate uptake. AT-treatment did not affect uptake of P52 but did decrease loss of p32 to the phosphate-free nutrient solutions after i t had been absorbed by the plants. The proportion of absorbed phosphate found in the stems and leaves of AT-treated plants was higher than in these organs in the control plants. This phosphate re…
has type: Thesis/Dissertation
is shown at: 10.14288/1.0106114
language: eng
degree discipline: Botany
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/40079