Some interrelations of root-rotting Basidiomycetes and soil-inhabiting Fungi imperfecti
department or school: Science, Faculty of
department or school: Botany, Department of
data provider: DSpace
degree campus: UBCV
creator: Pentland, Gertrude Draper
issued: 2011-10-28T16:26:35Z
issued: 1966
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: In the first of two parts of the investigation, the stimulation of Armiil aria mellea (Fr.) Quél., a common root-rotting fungus, by Aureobastdium pullulans (DeBary) Arnaud was studied. A. pullulans is an ubiquitous imperfect fungus, inhabiting soil and other substrates. It was shown to produce in culture a volatile, heat stable, neutral substance which stimulated the mycelial development, rhizomorph initiation and rhizomorph elongation of A. mellea. A. pullulans is the only microorganism reported so far to stimulate the growth of A. mellea. The effect of ethanol on the growth of A. mellea was similar to the effect of a cell-free filtrate from a liquid culture of A. pullulans. The cell-free filtrate was shown by gas chromatography to contain ethanol. Ethanol supplied daily as 50 ppm in a glucose-asparagine medium resulted in a ten-fold increase in the number of rhizomorphs produced by A. mellea. The effects of 3-indolylacetic acid, ɣ-(indole-3)-n-butyric acid, the sodium salt of 2,4-dichlorophenoxyacetic acid and tryptophane on the growth of A. mellea were tested, but the stimulatory effect of A. pullulans was not reproduced by them. One rhizomorph tip of A. mellea could develop an extensive rhizomorph system in autoclaved soil if the stimulatory substance produced by A. pullulans was available. In the second part of the investigation the effect of soil moisture and the related effect of some soil-inhabiting Fungi imperfecti on the spread through soil of the root-rotting Basidiomycete Coniopnora puteana (Schum. ex Fr.) Karst.were studied. A soil moisture level of 20 - 25% saturation was satisfactory for the growth of C. puteana in non-sterile soil. At this moisture level C. puteana was able to grow out from a small alder disc inoculum in the centre of a petri dish and invade alder discs at the periphery of the dish. In wetter conditions (50% saturation and higher) it was unable to grow out into the soil. In autoclaved soil the cptimum moisture level for the growth of…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/38365
note: The University of British Columbia FACULTY COT GEADUATE STUDIES PROGRAMME OF THE FINAL ORAL EXAMINATION FOR THE DEGREE OF DOCTOR OF PHILOSOPHY . of GERTRUDE.DRAPER PENTLAND B.A., The University of British Columbia, 1956 M.A., The University of British Columbia, 1959 THURSDAY, NOVEMBER 10, 1966 AT 3:30 P.M.-IN ROOM 3332, BIOLOGICAL SCIENCES BUILDING COMMITTEE IN CHARGE Chairman: I. McT. Cowan R. J . Bandoni T. M. C. Taylor J . E. Bier G. H. N. Towers C. A. Rowles E. B. Tregunna J . A. F. Gardner External Examiner: L . F. Roth Department of Botany and Plant Pathology Oregon State University . Corvallis, Oregon Research Supervisor: J . E. Bier SOME IWTERRELA,HONS OF ROOT~ROTTING BASIDIOMYCETES AND SOIL INHABITING FUNGI IMPERPECTI ABSTRACT In the f irs t of two parts of the investigation,, the stimulation of Armillaria mellea (Fr.) Quelog.a common root-rotting funguss by Aureobasidium. pullulans (DeBary) Arnaud was studied* A. pullulans is an ubiquitous imperfect fungus, inhabiting soil and other substrates„ It was shown to produce in culture a volatile^ heat stable,, neutral substance which stimulated the mycelial development., rhizomorph initiation and rhizomorph elongation of A<= mellea, h° PuHaLans is the only microorganism reported so far to stimulate the growth of A* mellea. The effect of ethanol on the growth of A„ mellea was similar to the effect of a cell=free fi ltrate from a l iquid culture of A, pullulans, The ce l l~£ree f i ltrate was shown by gas chromatography to contain ethanolo Ethanol supplied daily as 50 ppm in a glucose~asparagine medium resulted in a ten~fold increase in the number of rhizomorphs produced by Ao mellea. The effects of 3=indolylacetic acid s Tf-(indole-3) =n~butyric acid,, the sodium salt of 2?4=dichlorophen~ oxyacetic acid and tryptophane on the growth of A„ mellea were tested, but the stimulatory effect of A. pullulans was not reproduced by them. One^rhizomorph tip of A. mellea could develop an extensive rhizomorph system in autocl…
has type: Thesis/Dissertation
is shown at: 10.14288/1.0105478
language: eng
degree discipline: Botany
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/38365