Deuterium nuclear magnetic resonance study of water in model and biological membrane systems
department or school: Science, Faculty of
department or school: Physics and Astronomy, Department of
data provider: DSpace
degree campus: UBCV
creator: Wei, C.M.
issued: 2010-03-11T01:29:40Z
issued: 1979
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: A deuteron magnetic resonance study of water has been carried out in the lamellar phase of the egg yolk lecithin-water and outer membrane of E. Coli-water systems in excess water (abbreviated to EYL/EXCD₂O and EC/EXCD₂0, respectively) and egg yolk lecithin-water in 22% (by weight) water (EYL/22%WD₂0). Spectra of these systems were taken as a function of temperature, and their moments were calculated. Analysis of the integrated signal intensities revealed that the excess free (bulk) water in the EC/EXCD₂0 and EYL/EXCD₂O froze at -1°C and -2°C, respectively (pure D₂0 freezes at 4°C). The water from bilayers in the two excess water systems was frozen immediately after it was squeezed out, whereas the water squeezed out from bilayers in the EYL/22%WD₂0 remained unfrozen down to -10°C. All the squeezed out water in the EYL/22%WD₂0was frozen at -15°C. The bound water in that system was unfreezable in the region under study. The amount of water frozen out in the EYL/EXCD₂O at -2°C and in the EYL/EXCD₂O at -3°C was found to be approximately 85% of the total water content of the systems. The water frozen out in the EYL/22%WD₂0 at -15°C was determined to be 50% of the total water in that-;system. A minimum in the second moment vs temperature of the EYL/22%WD₂0 was observed and ascribed to the presence of isotropic free water squeezed out from the bilayers. Proton magnetic resonance results showed that there was no lipid phase transition in the EYL/22%WD₂0 in the region from -10°C to 48°C.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/21757
note: DEUTERIUM NUCLEAR MAGNETIC RESONANCE STUDY OF WATER IN MODEL AND BIOLOGICAL MEMBRANE SYSTEMS b y C. M. WEI A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE i n THE DEPARTMENT OF PHYSICS IF THE FACULTY OF GRADUATE STUDIES We a c c e p t t h i s t h e s i s as c o n f o r m i n g t o t h e r e q u i r e d s t a n d a r d THE UNIVERSITY OF B R I T I S H COLUMBIA October, 1979 (DfeC.M. Wei, 1979 In present ing th i s thes is in p a r t i a l fu l f i lment of the requirements for an advanced degree at the Univers i ty of B r i t i s h Columbia, I agree that the L ibrary shal l make it f ree l y ava i lab le for reference and study. I fur ther agree that permission for extensive copying of th is thes is for scho lar l y purposes may be granted by the Head of my Department or by h is representat ives . It is understood that copying or pub l i ca t ion of th is thes is fo r f i n a n c i a l gain sha l l not be allowed without my wri t ten permission. Department of The Univers i ty of B r i t i s h Columbia 2075 Wesbrook Place Vancouver, Canada V6T 1W5 G i i A b s t r a c t A deuteron magnetic resonance study of water has been c a r r i e d out i n the l a m e l l a r phase of the egg y o l k l e c i t h i n - w a t e r and outer membrane of E. Col i - w a t e r systems i n excess water (abbreviated to EYL/EXCD2O and EC/EXCD 20, r e s p e c t i v e l y ) and egg y o l k l e c i t h i n - w a t e r i n 2 2 % (by weight) water (EYL/22%WD20). Spectra of these systems were taken as a f u n c t i o n of temperature, and t h e i r moments were c a l c u l a t e d . A n a l y s i s of the i n t e g r a t e d s i g n a l i n t e n s i t i e s revealed that the excess free (bulk) water i n the EC/EXCD 20 and EYL/EXCD^O froze a t -1°C and -2°C, r e s p e c t i v e l y (pure _ 2© freezes at 4°C). The water from b i l a y e r s i n the two excess water systems was frozen immediately a f t e r i t was squeezed out, whereas the water sque…
has type: Thesis/Dissertation
is shown at: 10.14288/1.0103713
language: eng
degree discipline: Physics
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/21757